1cxu: Difference between revisions

Revision as of 13:13, 2 May 2008

1.42A RESOLUTION ASV INTEGRASE CORE DOMAIN FROM CITRATE

OverviewOverview

Six crystal structures of the core domain of integrase (IN) from avian sarcoma virus (ASV) and its active-site derivative containing an Asp64 --> Asn substitution have been solved at atomic resolution ranging 1.02-1.42 A. The high-quality data provide new structural information about the active site of the enzyme and clarify previous inconsistencies in the description of this fragment. The very high resolution of the data and excellent quality of the refined models explain the dynamic properties of IN and the multiple conformations of its disordered residues. They also allow an accurate description of the solvent structure and help to locate other molecules bound to the enzyme. A detailed analysis of the flexible active-site region, in particular the loop formed by residues 144-154, suggests conformational changes which may be associated with substrate binding and enzymatic activity. The pH-dependent conformational changes of the active-site loop correlates with the pH vs activity profile observed for ASV IN.

About this StructureAbout this Structure

1CXU is a Single protein structure of sequence from Avian sarcoma virus. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution structures of the core domain of avian sarcoma virus integrase and its D64N mutant., Lubkowski J, Dauter Z, Yang F, Alexandratos J, Merkel G, Skalka AM, Wlodawer A, Biochemistry. 1999 Oct 12;38(41):13512-22. PMID:10521258 Page seeded by OCA on Fri May 2 13:13:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1cxu.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1cxu |SIZE=350|CAPTION= <scene name='initialview01'>1cxu</scene>, resolution 1.42&Aring;
+The line below this paragraph, containing "STRUCTURE_1cxu", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
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-|GENE=
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-|DOMAIN=
+{{STRUCTURE_1cxu|  PDB=1cxu  |  SCENE=  }}
-|RELATEDENTRY=[[1asv|1ASV]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxu OCA], [http://www.ebi.ac.uk/pdbsum/1cxu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cxu RCSB]</span>
-}}
 '''1.42A RESOLUTION ASV INTEGRASE CORE DOMAIN FROM CITRATE'''
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 [[Category: Wlodawer, A.]]
 [[Category: Yang, F.]]
-[[Category: mixed beta-sheet surrounded by alpha-helice]]
+[[Category: Mixed beta-sheet surrounded by alpha-helice]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:13:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:38 2008''