1cxv: Difference between revisions

Revision as of 13:13, 2 May 2008

STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)

OverviewOverview

The matrix metalloproteinases are crucial in the physiological and pathological degradation of the mammalian extracellular matrix, including breast tumours, and osteoarthritic cartilage. These enzymes are classified according to their matrix substrate specificity. Collagenase-3 (MMP-13) is a member of this family and preferentially cleaves type II collagen, cartilage, fibronectin and aggrecan. Collagenase-3 is normally expressed in hypertrophic chondrocytes, periosteal cells, and osteoblasts during bone development. The structure of the catalytic domain of recombinant mouse collagenase-3, complexed to the hydroxamate inhibitor (RS-113456), is reported at 2.0 A resolution. Molecular replacement and weak phasing information from a single derivative determined the structure. Neither molecular replacement nor derivative methods had a sufficient radius of convergence to yield a refinable structure. The structure illuminates the atomic zinc ion interactions with functional groups in the active site, emphasizing zinc ligation and the very voluminous hydrophobic P1' group for the inhibitor potency. The structure provides insight into the specificity of this enzyme, facilitating design of specific inhibitors to target various diseases.

About this StructureAbout this Structure

1CXV is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of recombinant mouse collagenase-3 (MMP-13)., Botos I, Meyer E, Swanson SM, Lemaitre V, Eeckhout Y, Meyer EF, J Mol Biol. 1999 Oct 1;292(4):837-44. PMID:10525409 Page seeded by OCA on Fri May 2 13:13:33 2008

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OCA

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 [[Image:1cxv.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1cxv", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxv OCA], [http://www.ebi.ac.uk/pdbsum/1cxv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cxv RCSB]</span>
-}}
 '''STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)'''
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 [[Category: Meyer, E F.]]
 [[Category: Swanson, S M.]]
-[[Category: collagen degradation]]
+[[Category: Collagen degradation]]
-[[Category: glycoprotein]]
+[[Category: Glycoprotein]]
-[[Category: metalloprotease]]
+[[Category: Metalloprotease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:13:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:37 2008''