5y87: Difference between revisions
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==Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme== | ==Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme== | ||
<StructureSection load='5y87' size='340' side='right' caption='[[5y87]], [[Resolution|resolution]] 2.13Å' scene=''> | <StructureSection load='5y87' size='340' side='right'caption='[[5y87]], [[Resolution|resolution]] 2.13Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5y87]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y87 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5y87]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y87 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y87 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y87 OCA], [http://pdbe.org/5y87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y87 RCSB], [http://www.ebi.ac.uk/pdbsum/5y87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y87 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5y87" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5y87" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Ribozyme|Ribozyme]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Micura, R L]] | [[Category: Micura, R L]] | ||
[[Category: Ren, A]] | [[Category: Ren, A]] | ||
Revision as of 12:13, 21 October 2020
Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister RibozymeStructure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme
Structural highlights
Publication Abstract from PubMedHere we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg2+ cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg2+ ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme. Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme.,Zheng L, Mairhofer E, Teplova M, Zhang Y, Ma J, Patel DJ, Micura R, Ren A Nat Commun. 2017 Oct 30;8(1):1180. doi: 10.1038/s41467-017-01276-y. PMID:29081514[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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