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'''CATALYTIC ANTIBODY 7C8 COMPLEX''' | '''CATALYTIC ANTIBODY 7C8 COMPLEX''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT8 OCA]. | |||
==Reference== | ==Reference== | ||
Diverse structural solutions to catalysis in a family of antibodies., Gigant B, Tsumuraya T, Fujii I, Knossow M, Structure. 1999 Nov 15;7(11):1385-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10574796 10574796] | Diverse structural solutions to catalysis in a family of antibodies., Gigant B, Tsumuraya T, Fujii I, Knossow M, Structure. 1999 Nov 15;7(11):1385-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10574796 10574796] | ||
[[Category: Fujii, I.]] | [[Category: Fujii, I.]] | ||
[[Category: Gigant, B.]] | [[Category: Gigant, B.]] | ||
[[Category: Knossow, M.]] | [[Category: Knossow, M.]] | ||
[[Category: Tsumuraya, T.]] | [[Category: Tsumuraya, T.]] | ||
[[Category: | [[Category: Abzyme transition state analog]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:05:22 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 13:05, 2 May 2008
CATALYTIC ANTIBODY 7C8 COMPLEX
OverviewOverview
BACKGROUND: Small organic molecules coupled to a carrier protein elicit an antibody response on immunisation. The diversity of this response has been found to be very narrow in several cases. Some antibodies also catalyse chemical reactions. Such catalytic antibodies are usually identified among those that bind tightly to an analogue of the transition state (TSA) of the relevant reaction; therefore, catalytic antibodies are also thought to have restricted diversity. To further characterise this diversity, we investigated the structure and biochemistry of the catalytic antibody 7C8, one of the most efficient of those which enhance the hydrolysis of chloramphenicol esters, and compared it to the other catalytic antibodies elicited in the same immunisation. RESULTS: The structure of a complex of the 7C8 antibody Fab fragment with the hapten TSA used to elicit it was determined at 2.2 A resolution. Structural comparison with another catalytic antibody (6D9) raised against the same hapten revealed that the two antibodies use different binding modes. Furthermore, whereas 6D9 catalyses hydrolysis solely by transition-state stabilisation, data on 7C8 show that the two antibodies use mechanisms where the catalytic residue, substrate specificity and rate-limiting step differ. CONCLUSIONS: Our results demonstrate that substantial diversity may be present among antibodies catalysing the same reaction. Therefore, some of these antibodies represent different starting points for mutagenesis aimed at boosting their activity. This increases the chance of obtaining more proficient catalysts and provides opportunities for tailoring catalysts with different specificities.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Diverse structural solutions to catalysis in a family of antibodies., Gigant B, Tsumuraya T, Fujii I, Knossow M, Structure. 1999 Nov 15;7(11):1385-93. PMID:10574796 Page seeded by OCA on Fri May 2 13:05:22 2008