1cn0: Difference between revisions

Revision as of 12:54, 2 May 2008

CRYSTAL STRUCTURE OF D(ACCCT)

OverviewOverview

The crystal structure of d(ACCCT), solved by molecular replacement, shows a four-stranded i-motif conformation, where two parallel duplexes intercalate with one another in opposite orientations. Each duplex is stabilized by hemi-protonated C-C+ base pairing between parallel strands, and a string of water molecules bridge the cytosine N4 atoms to phosphate O atoms. This structure of d(ACCCT) shows examples of reversed Hoogsteen and Watson-Crick base pairing in both intermolecular and intramolecular manners to stabilize the tetraplex. Noticeably, the four-stranded complex is further stabilized at one end by a three-base hydrogen-bonding network, in which two adenines and a thymine form four hydrogen bonds via a reverse Hoogsteen and an asymmetric adenine-adenine base pairing. The structure of d(ACCCT) shows a similar local structure to that found in the d(TAA) part of the crystal structure of d(TAACCC) and provides further structural evidence that these base arrangements are essential for stabilizing these novel DNA super-secondary structures.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Stabilization of the i-motif by intramolecular adenine-adenine-thymine base triple in the structure of d(ACCCT)., Weil J, Min T, Yang C, Wang S, Sutherland C, Sinha N, Kang C, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):422-9. PMID:10089350 Page seeded by OCA on Fri May 2 12:54:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1cn0.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1cn0", creates the "Structure Box" on the page.
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cn0 OCA], [http://www.ebi.ac.uk/pdbsum/1cn0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cn0 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF D(ACCCT)'''
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 ==About this Structure==
-CN0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CN0 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CN0 OCA].
 ==Reference==
 Stabilization of the i-motif by intramolecular adenine-adenine-thymine base triple in the structure of d(ACCCT)., Weil J, Min T, Yang C, Wang S, Sutherland C, Sinha N, Kang C, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):422-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089350 10089350]
-[[Category: Protein complex]]
 [[Category: Cheng, Y.]]
 [[Category: Kang, C.]]
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 [[Category: Wang, S.]]
 [[Category: Weil, J.]]
-[[Category: i-motif]]
+[[Category: I-motif]]
-[[Category: telomere]]
+[[Category: Telomere]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:54:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:24:44 2008''