1clq: Difference between revisions

Revision as of 12:52, 2 May 2008

CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION

OverviewOverview

We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.

About this StructureAbout this Structure

1CLQ is a Single protein structure of sequence from Enterobacteria phage rb69. The following page contains interesting information on the relation of 1CLQ with [DNA Polymerase]. Full crystallographic information is available from OCA.

ReferenceReference

Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:10535734 Page seeded by OCA on Fri May 2 12:52:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1clq.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1clq |SIZE=350|CAPTION= <scene name='initialview01'>1clq</scene>, resolution 2.70&Aring;
+The line below this paragraph, containing "STRUCTURE_1clq", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= GENE 43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69])
+-->
-|DOMAIN=
+{{STRUCTURE_1clq|  PDB=1clq  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clq OCA], [http://www.ebi.ac.uk/pdbsum/1clq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1clq RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION'''
@@ Line 29: / Line 26: @@
 [[Category: Shamoo, Y.]]
 [[Category: Steitz, T A.]]
-[[Category: dna polymerase]]
+[[Category: Dna polymerase]]
-[[Category: editing]]
+[[Category: Editing]]
-[[Category: gp43]]
+[[Category: Gp43]]
-[[Category: proofreading]]
+[[Category: Proofreading]]
-[[Category: replication]]
+[[Category: Replication]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:52:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:24:05 2008''