5odl: Difference between revisions
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==Single-stranded DNA-binding protein from bacteriophage Enc34 in complex with ssDNA== | ==Single-stranded DNA-binding protein from bacteriophage Enc34 in complex with ssDNA== | ||
<StructureSection load='5odl' size='340' side='right' caption='[[5odl]], [[Resolution|resolution]] 1.56Å' scene=''> | <StructureSection load='5odl' size='340' side='right'caption='[[5odl]], [[Resolution|resolution]] 1.56Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5odl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_phage_enc34 Enterobacter phage enc34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODL OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5odl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_phage_enc34 Enterobacter phage enc34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ODL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5odj|5odj]], [[5odk|5odk]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5odj|5odj]], [[5odk|5odk]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5odl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odl OCA], [http://pdbe.org/5odl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5odl RCSB], [http://www.ebi.ac.uk/pdbsum/5odl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5odl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5odl" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5odl" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Enterobacter phage enc34]] | [[Category: Enterobacter phage enc34]] | ||
[[Category: Large Structures]] | |||
[[Category: Cernooka, E]] | [[Category: Cernooka, E]] | ||
[[Category: Kazaks, A]] | [[Category: Kazaks, A]] | ||
Revision as of 15:21, 26 August 2020
Single-stranded DNA-binding protein from bacteriophage Enc34 in complex with ssDNASingle-stranded DNA-binding protein from bacteriophage Enc34 in complex with ssDNA
Structural highlights
Publication Abstract from PubMedModern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless conserved among distantly related phages. Here, we report the crystal structure of a conserved Enc34 replication protein ORF6 which contains a domain of unknown function DUF2815. Despite the low (~15%) sequence identity, the Enc34 ORF6 structurally resembles the gene 2.5 protein from bacteriophage T7, and likewise is a single-stranded DNA (ssDNA)-binding protein (SSB) that consists of a variation of the oligosaccharide/oligonucleotide-binding (OB)-fold and an unstructured C-terminal segment. We further report the crystal structure of a C-terminally truncated ORF6 in complex with an ssDNA oligonucleotide that reveals a DNA-binding mode involving two aromatic stacks and multiple electrostatic interactions, with implications for a common ssDNA recognition mechanism for all T7-type SSBs. Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.,Cernooka E, Rumnieks J, Tars K, Kazaks A Sci Rep. 2017 Nov 14;7(1):15529. doi: 10.1038/s41598-017-15774-y. PMID:29138440[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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