5nci: Difference between revisions
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==GriE in complex with cobalt, alpha-ketoglutarate and l-leucine== | ==GriE in complex with cobalt, alpha-ketoglutarate and l-leucine== | ||
<StructureSection load='5nci' size='340' side='right' caption='[[5nci]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='5nci' size='340' side='right'caption='[[5nci]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5nci]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCI OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5nci]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NCI FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nci OCA], [http://pdbe.org/5nci PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nci RCSB], [http://www.ebi.ac.uk/pdbsum/5nci PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nci ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5nci" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5nci" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Hydroxylases 3D structures|Hydroxylases 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Blankenfeldt, W]] | [[Category: Blankenfeldt, W]] | ||
[[Category: Lukat, P]] | [[Category: Lukat, P]] | ||
Revision as of 15:02, 26 August 2020
GriE in complex with cobalt, alpha-ketoglutarate and l-leucineGriE in complex with cobalt, alpha-ketoglutarate and l-leucine
Structural highlights
Publication Abstract from PubMedGriselimycins (GMs) are depsidecapeptides with superb anti-tuberculosis activity. They contain up to three (2S,4R)-4-methyl-prolines (4-MePro), of which one blocks oxidative degradation and increases metabolic stability in animal models. The natural congener with this substitution is only a minor component in fermentation cultures. We showed that this product can be significantly increased by feeding the reaction with 4-MePro and we investigated the molecular basis of 4-MePro biosynthesis and incorporation. We identified the GM biosynthetic gene cluster as encoding a nonribosomal peptide synthetase and a sub-operon for 4-MePro formation. Using heterologous expression, gene inactivation, and in vitro experiments, we showed that 4-MePro is generated by leucine hydroxylation, oxidation to an aldehyde, and ring closure with subsequent reduction. The crystal structures of the leucine hydroxylase GriE have been determined in complex with substrates and products, providing insight into the stereospecificity of the reaction. Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.,Lukat P, Katsuyama Y, Wenzel S, Binz T, Konig C, Blankenfeldt W, Bronstrup M, Muller R Chem Sci. 2017 Nov 1;8(11):7521-7527. doi: 10.1039/c7sc02622f. Epub 2017 Aug 3. PMID:29163906[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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