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==Cryatal stucture of AofleA from Arthrobotrys oligospora in complex with methylated L-fucose== | ==Cryatal stucture of AofleA from Arthrobotrys oligospora in complex with methylated L-fucose== | ||
<StructureSection load='7c39' size='340' side='right'caption='[[7c39]]' scene=''> | <StructureSection load='7c39' size='340' side='right'caption='[[7c39]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C39 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C39 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7c39]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Artoa Artoa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C39 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C39 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c39 OCA], [http://pdbe.org/7c39 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c39 RCSB], [http://www.ebi.ac.uk/pdbsum/7c39 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c39 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MFU:ALPHA-L-METHYL-FUCOSE'>MFU</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[7c37|7c37]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AOL_s00076g540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=756982 ARTOA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c39 OCA], [http://pdbe.org/7c39 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c39 RCSB], [http://www.ebi.ac.uk/pdbsum/7c39 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c39 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fungal lectin can bind specific carbohydrate structures of the host and work in recognition and adhesion or as a toxic factor. AofleA, as a fucose-specific lectin from widely studied nematode predatory fungus Arthrobotrys oligospora, possibly plays a key role in the event of capturing nematodes, but the mechanism remains unknown. Here we report the crystal structure of AofleA, which exists as a homodimer with each subunit folds as a six-bladed beta-propeller. Our structural and biological results revealed that three of the six putative binding sites of AofleA had fucose-binding abilities. In addition, we found that AofleA could bind to the pharynx and intestine of the nematode in a fucose-binding-dependent manner. Our results facilitate the understanding of the mechanism that fucose-specific lectin mediates fungi-nematodes interaction, and provide structural information for the development of potential applications of AofleA. | |||
Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.,Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064<ref>PMID:32698064</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7c39" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Artoa]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cheng X]] | [[Category: Cheng, X]] | ||
[[Category: Liu M]] | [[Category: Liu, M]] | ||
[[Category: Wang J]] | [[Category: Wang, J]] | ||
[[Category: Wang M]] | [[Category: Wang, M]] | ||
[[Category: Zhang M]] | [[Category: Zhang, M]] | ||
[[Category: Arthrobotrys oligospora]] | |||
[[Category: Fucose-specific lectin]] | |||
[[Category: Nematode-trapping fungi]] | |||
[[Category: Sugar binding protein]] | |||
Revision as of 09:01, 5 August 2020
Cryatal stucture of AofleA from Arthrobotrys oligospora in complex with methylated L-fucoseCryatal stucture of AofleA from Arthrobotrys oligospora in complex with methylated L-fucose
Structural highlights
Publication Abstract from PubMedFungal lectin can bind specific carbohydrate structures of the host and work in recognition and adhesion or as a toxic factor. AofleA, as a fucose-specific lectin from widely studied nematode predatory fungus Arthrobotrys oligospora, possibly plays a key role in the event of capturing nematodes, but the mechanism remains unknown. Here we report the crystal structure of AofleA, which exists as a homodimer with each subunit folds as a six-bladed beta-propeller. Our structural and biological results revealed that three of the six putative binding sites of AofleA had fucose-binding abilities. In addition, we found that AofleA could bind to the pharynx and intestine of the nematode in a fucose-binding-dependent manner. Our results facilitate the understanding of the mechanism that fucose-specific lectin mediates fungi-nematodes interaction, and provide structural information for the development of potential applications of AofleA. Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.,Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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