2md0: Difference between revisions
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==Solution structure of ShK-like immunomodulatory peptide from Ancylostoma caninum (hookworm)== | ==Solution structure of ShK-like immunomodulatory peptide from Ancylostoma caninum (hookworm)== | ||
<StructureSection load='2md0' size='340' side='right' caption='[[2md0]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | <StructureSection load='2md0' size='340' side='right'caption='[[2md0]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2md0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ancca Ancca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MD0 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2md0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ancca Ancca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MD0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MD0 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2md0 OCA], [http://pdbe.org/2md0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2md0 RCSB], [http://www.ebi.ac.uk/pdbsum/2md0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2md0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Ancca]] | [[Category: Ancca]] | ||
[[Category: Large Structures]] | |||
[[Category: Chandy, G K]] | [[Category: Chandy, G K]] | ||
[[Category: Chang, S C]] | [[Category: Chang, S C]] | ||
Revision as of 14:52, 29 July 2020
Solution structure of ShK-like immunomodulatory peptide from Ancylostoma caninum (hookworm)Solution structure of ShK-like immunomodulatory peptide from Ancylostoma caninum (hookworm)
Structural highlights
Publication Abstract from PubMedThe voltage-gated potassium (Kv) 1.3 channel is widely regarded as a therapeutic target for immunomodulation in autoimmune diseases. ShK-186, a selective inhibitor of Kv1.3 channels, ameliorates autoimmune diseases in rodent models, and human phase 1 trials of this agent in healthy volunteers have been completed. In this study, we identified and characterized a large family of Stichodactyla helianthus toxin (ShK)-related peptides in parasitic worms. Based on phylogenetic analysis, 2 worm peptides were selected for study: AcK1, a 51-residue peptide expressed in the anterior secretory glands of the dog-infecting hookworm Ancylostoma caninum and the human-infecting hookworm Ancylostoma ceylanicum, and BmK1, the C-terminal domain of a metalloprotease from the filarial worm Brugia malayi. These peptides in solution adopt helical structures closely resembling that of ShK. At doses in the nanomolar-micromolar range, they block native Kv1.3 in human T cells and cloned Kv1.3 stably expressed in L929 mouse fibroblasts. They preferentially suppress the proliferation of rat CCR7- effector memory T cells without affecting naive and central memory subsets and inhibit the delayed-type hypersensitivity (DTH) response caused by skin-homing effector memory T cells in rats. Further, they suppress IFNgamma production by human T lymphocytes. ShK-related peptides in parasitic worms may contribute to the potential beneficial effects of probiotic parasitic worm therapy in human autoimmune diseases.-Chhabra, S., Chang, S. C., Nguyen, H. M., Huq, R., Tanner, M. R., Londono, L. M., Estrada, R., Dhawan, V., Chauhan, S., Upadhyay, S. K., Gindin, M., Hotez, P. J., Valenzuela, J. G., Mohanty, B., Swarbrick, J. D., Wulff, H., Iadonato, S. P., Gutman, G. A., Beeton, C., Pennington, M. W., Norton, R. S., Chandy, K. G. Kv1.3 channel-blocking immunomodulatory peptides from parasitic worms: implications for autoimmune diseases. Kv1.3 channel-blocking immunomodulatory peptides from parasitic worms: implications for autoimmune diseases.,Chhabra S, Chang SC, Nguyen HM, Huq R, Tanner MR, Londono LM, Estrada R, Dhawan V, Chauhan S, Upadhyay SK, Gindin M, Hotez PJ, Valenzuela JG, Mohanty B, Swarbrick JD, Wulff H, Iadonato SP, Gutman GA, Beeton C, Pennington MW, Norton RS, Chandy KG FASEB J. 2014 Jun 2. pii: fj.14-251967. PMID:24891519[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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