6y98: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose== | ==Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose== | ||
<StructureSection load='6y98' size='340' side='right'caption='[[6y98]]' scene=''> | <StructureSection load='6y98' size='340' side='right'caption='[[6y98]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y98 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y98 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6y98]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canga Canga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y98 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y98 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y98 OCA], [http://pdbe.org/6y98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y98 RCSB], [http://www.ebi.ac.uk/pdbsum/6y98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y98 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPA9, CAGL0A01366g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284593 CANGA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y98 OCA], [http://pdbe.org/6y98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y98 RCSB], [http://www.ebi.ac.uk/pdbsum/6y98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y98 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
For host-cell interaction, the human fungal pathogen Candida glabrata harbors a large family of more than 20 cell wall attached epithelial adhesins (Epas). Epa family members are lectins with binding pockets containing several conserved and variable structural hot spots, which were implicated in mediating functional diversity. In this study, we have performed an elaborate structure-based mutational analysis of numerous Epa paralogs, in order to generally determine the role of diverse structural hot spots in conferring host cell binding and ligand binding specificity. Our study reveals that several conserved structural motifs contribute to efficient host cell binding. Moreover, our directed motif exchange experiments reveal that the variable loop CBL2 is key for programming ligand binding specificity, albeit with limited predictability. In contrast, we find that the variable loop L1 affects host cell binding without significantly influencing specificity of ligand binding. Our data strongly suggest, that variation of numerous structural hot spots in the ligand binding pocket of Epa proteins is a main driver of their functional diversification and evolution. | |||
Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.,Hoffmann D, Diderrich R, Reithofer V, Friederichs S, Kock M, Essen LO, Mosch HU J Biol Chem. 2020 Jul 15. pii: RA120.013968. doi: 10.1074/jbc.RA120.013968. PMID:32669365<ref>PMID:32669365</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6y98" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Canga]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Diderrich R]] | [[Category: Diderrich, R]] | ||
[[Category: Essen L | [[Category: Essen, L O]] | ||
[[Category: Friederichs S]] | [[Category: Friederichs, S]] | ||
[[Category: Hoffmann D]] | [[Category: Hoffmann, D]] | ||
[[Category: Kock M]] | [[Category: Kock, M]] | ||
[[Category: Moesch H | [[Category: Moesch, H U]] | ||
[[Category: Reithofer V]] | [[Category: Reithofer, V]] | ||
[[Category: Cell adhesion]] | |||
[[Category: Epithelial adhesin]] | |||
Revision as of 14:40, 29 July 2020
Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactoseCrystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose
Structural highlights
Publication Abstract from PubMedFor host-cell interaction, the human fungal pathogen Candida glabrata harbors a large family of more than 20 cell wall attached epithelial adhesins (Epas). Epa family members are lectins with binding pockets containing several conserved and variable structural hot spots, which were implicated in mediating functional diversity. In this study, we have performed an elaborate structure-based mutational analysis of numerous Epa paralogs, in order to generally determine the role of diverse structural hot spots in conferring host cell binding and ligand binding specificity. Our study reveals that several conserved structural motifs contribute to efficient host cell binding. Moreover, our directed motif exchange experiments reveal that the variable loop CBL2 is key for programming ligand binding specificity, albeit with limited predictability. In contrast, we find that the variable loop L1 affects host cell binding without significantly influencing specificity of ligand binding. Our data strongly suggest, that variation of numerous structural hot spots in the ligand binding pocket of Epa proteins is a main driver of their functional diversification and evolution. Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.,Hoffmann D, Diderrich R, Reithofer V, Friederichs S, Kock M, Essen LO, Mosch HU J Biol Chem. 2020 Jul 15. pii: RA120.013968. doi: 10.1074/jbc.RA120.013968. PMID:32669365[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||