1c9s: Difference between revisions

Revision as of 12:30, 2 May 2008

CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES

OverviewOverview

The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes of several bacilli by binding single-stranded RNA. The binding sequence is composed of eleven triplet repeats, predominantly GAG, separated by two or three non-conserved nucleotides. Here we present the crystal structure of a complex of TRAP and a 53-base single-stranded RNA containing eleven GAG triplets, revealing that each triplet is accommodated in a binding pocket formed by beta-strands. In the complex, the RNA has an extended structure without any base-pairing and binds to the protein mostly by specific protein-base interactions. Eleven binding pockets on the circular TRAP 11-mer form a belt with a diameter of about 80 A. This simple but elegant mechanism of arresting the RNA segment by encircling it around a protein disk is applicable to both transcription, when TRAP binds the nascent RNA, and to translation, when TRAP binds the same sequence within a non-coding leader region of the messenger RNA.

About this StructureAbout this Structure

1C9S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA., Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen X, Gollnick P, Nature. 1999 Sep 16;401(6750):235-42. PMID:10499579 Page seeded by OCA on Fri May 2 12:30:15 2008

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OCA

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 [[Image:1c9s.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1c9s", creates the "Structure Box" on the page.
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9s OCA], [http://www.ebi.ac.uk/pdbsum/1c9s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c9s RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES'''
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 [[Category: Gollnick, P.]]
 [[Category: Greaves, R B.]]
-[[Category: protein-rna complex]]
+[[Category: Protein-rna complex]]
-[[Category: single stranded rna]]
+[[Category: Single stranded rna]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: trap]]
+[[Category: Trap]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:30:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:17:31 2008''