6wc7: Difference between revisions
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== | ==Acyl carrier protein (ACP) domain bound to dehydratase (DH) domain in fungal fatty acid synthase (FAS)== | ||
<StructureSection load='6wc7' size='340' side='right'caption='[[6wc7]]' scene=''> | <StructureSection load='6wc7' size='340' side='right'caption='[[6wc7]], [[Resolution|resolution]] 5.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WC7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WC7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6wc7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WC7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WC7 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wc7 OCA], [http://pdbe.org/6wc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wc7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wc7 ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FAS1, YKL182W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), FAS2, YPL231W, P1409 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wc7 OCA], [http://pdbe.org/6wc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wc7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wc7 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/FAS1_YEAST FAS1_YEAST]] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase. [[http://www.uniprot.org/uniprot/FAS2_YEAST FAS2_YEAST]] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. This subunit coordinates the binding of the six beta subunits to the enzyme complex.[HAMAP-Rule:MF_00101] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The acyl carrier protein (ACP) domain shuttles substrates and reaction intermediates in type I fungal fatty acid synthases via transient protein-protein interactions. Here, using electron cryo-microscopy (cryoEM), we report the structure of a fungal FAS stalled at the dehydration reaction, which precedes the final enoyl reduction in the fatty acid biosynthesis cycle. This conformation revealed multiple contact sites between ACP and the dehydratase (DH) and enoyl reductase (ER) domains that occluded the ACP binding to the adjacent ER domain. Our data suggests a minimal path from the DH to the ER reaction site that requires minute changes in the coordinates of the structured N- and C- termini of the ACP domain. | |||
Steric occlusion regulates proximal interactions of acyl carrier protein domain in fungal fatty acid synthase.,Lou JW, Mazhab-Jafari MT Commun Biol. 2020 May 29;3(1):274. doi: 10.1038/s42003-020-0997-y. PMID:32471977<ref>PMID:32471977</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6wc7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 18824]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Lou, J W]] | ||
[[Category: Mazhab-Jafari, M T]] | |||
[[Category: Acyl carrier protein]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Dehydratase]] | |||
[[Category: Fatty acid synthase]] | |||
[[Category: Transferase]] | |||