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==Crystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose== | ==Crystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose== | ||
<StructureSection load='6ter' size='340' side='right'caption='[[6ter]]' scene=''> | <StructureSection load='6ter' size='340' side='right'caption='[[6ter]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TER OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TER FirstGlance]. <br> | <table><tr><td colspan='2'>[[6ter]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TER OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TER FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ter FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ter OCA], [http://pdbe.org/6ter PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ter RCSB], [http://www.ebi.ac.uk/pdbsum/6ter PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ter ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Blon_2062 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ter FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ter OCA], [http://pdbe.org/6ter PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ter RCSB], [http://www.ebi.ac.uk/pdbsum/6ter PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ter ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs. | |||
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.,Keenan T, Parmeggiani F, Malassis J, Fontenelle CQ, Vendeville JB, Offen W, Both P, Huang K, Marchesi A, Heyam A, Young C, Charnock SJ, Davies GJ, Linclau B, Flitsch SL, Fascione MA Cell Chem Biol. 2020 Jun 30. pii: S2451-9456(20)30228-2. doi:, 10.1016/j.chembiol.2020.06.005. PMID:32619452<ref>PMID:32619452</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6ter" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bifls]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Both P]] | [[Category: Both, P]] | ||
[[Category: Charnock S]] | [[Category: Charnock, S]] | ||
[[Category: Davies | [[Category: Davies, G J]] | ||
[[Category: Fascione | [[Category: Fascione, M A]] | ||
[[Category: Flitsch | [[Category: Flitsch, S L]] | ||
[[Category: Fontenelle | [[Category: Fontenelle, C Q]] | ||
[[Category: Heyam A]] | [[Category: Heyam, A]] | ||
[[Category: Huang K]] | [[Category: Huang, K]] | ||
[[Category: Keenan T]] | [[Category: Keenan, T]] | ||
[[Category: Linclau B]] | [[Category: Linclau, B]] | ||
[[Category: Malassis J]] | [[Category: Malassis, J]] | ||
[[Category: Marchesi A]] | [[Category: Marchesi, A]] | ||
[[Category: Offen | [[Category: Offen, W A]] | ||
[[Category: Parmeggiani F]] | [[Category: Parmeggiani, F]] | ||
[[Category: Vendeville J]] | [[Category: Vendeville, J]] | ||
[[Category: Young C]] | [[Category: Young, C]] | ||
[[Category: Complex]] | |||
[[Category: Gal]] | |||
[[Category: Galactokinase]] | |||
[[Category: Transferase]] | |||
Revision as of 14:31, 22 July 2020
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with GalactoseCrystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose
Structural highlights
Publication Abstract from PubMedFluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs. Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.,Keenan T, Parmeggiani F, Malassis J, Fontenelle CQ, Vendeville JB, Offen W, Both P, Huang K, Marchesi A, Heyam A, Young C, Charnock SJ, Davies GJ, Linclau B, Flitsch SL, Fascione MA Cell Chem Biol. 2020 Jun 30. pii: S2451-9456(20)30228-2. doi:, 10.1016/j.chembiol.2020.06.005. PMID:32619452[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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