6hqv: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 1: Line 1:


==Pentafunctional AROM Complex from Chaetomium thermophilum==
==Pentafunctional AROM Complex from Chaetomium thermophilum==
<StructureSection load='6hqv' size='340' side='right'caption='[[6hqv]]' scene=''>
<StructureSection load='6hqv' size='340' side='right'caption='[[6hqv]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HQV FirstGlance]. <br>
<table><tr><td colspan='2'>[[6hqv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HQV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HQV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqv OCA], [http://pdbe.org/6hqv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqv RCSB], [http://www.ebi.ac.uk/pdbsum/6hqv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqv ProSAT]</span></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DS:(4S,5R)-4,5-DIHYDROXY-3-OXOCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>3DS</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0008860 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hqv OCA], [http://pdbe.org/6hqv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hqv RCSB], [http://www.ebi.ac.uk/pdbsum/6hqv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hqv ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/G0S061_CHATD G0S061_CHATD]] The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.[HAMAP-Rule:MF_03143][PIRNR:PIRNR000514][SAAS:SAAS00712173]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The AROM complex is a multifunctional metabolic machine with ten enzymatic domains catalyzing the five central steps of the shikimate pathway in fungi and protists. We determined its crystal structure and catalytic behavior, and elucidated its conformational space using a combination of experimental and computational approaches. We derived this space in an elementary approach, exploiting an abundance of conformational information from its monofunctional homologs in the Protein Data Bank. It demonstrates how AROM is optimized for spatial compactness while allowing for unrestricted conformational transitions and a decoupled functioning of its individual enzymatic entities. With this architecture, AROM poses a tractable test case for the effects of active site proximity on the efficiency of both natural metabolic systems and biotechnological pathway optimization approaches. We show that a mere colocalization of enzymes is not sufficient to yield a detectable improvement of metabolic throughput.
Architecture and functional dynamics of the pentafunctional AROM complex.,Arora Veraszto H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD Nat Chem Biol. 2020 Jul 6. pii: 10.1038/s41589-020-0587-9. doi:, 10.1038/s41589-020-0587-9. PMID:32632294<ref>PMID:32632294</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hqv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chatd]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Arora Verasto H]]
[[Category: Hartmann, M D]]
[[Category: Hartmann MD]]
[[Category: Verasto, H Arora]]
[[Category: 3-dehydroquinate dehydratase]]
[[Category: 3-dehydroquinate synthase]]
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
[[Category: 5-enolpyruvylshikimate-3-phosphate synthase]]
[[Category: Aromatic amino acid biosynthesis]]
[[Category: Biosynthetic protein]]
[[Category: Multifunctional enzyme]]
[[Category: Shikimate dehydrogenase]]
[[Category: Shikimate kinase]]
[[Category: Shikimate pathway]]

Latest revision as of 14:18, 22 July 2020

Pentafunctional AROM Complex from Chaetomium thermophilumPentafunctional AROM Complex from Chaetomium thermophilum

Structural highlights

6hqv is a 2 chain structure with sequence from Chatd. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , , , ,
NonStd Res:
Gene:CTHT_0008860 (CHATD)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[G0S061_CHATD] The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.[HAMAP-Rule:MF_03143][PIRNR:PIRNR000514][SAAS:SAAS00712173]

Publication Abstract from PubMed

The AROM complex is a multifunctional metabolic machine with ten enzymatic domains catalyzing the five central steps of the shikimate pathway in fungi and protists. We determined its crystal structure and catalytic behavior, and elucidated its conformational space using a combination of experimental and computational approaches. We derived this space in an elementary approach, exploiting an abundance of conformational information from its monofunctional homologs in the Protein Data Bank. It demonstrates how AROM is optimized for spatial compactness while allowing for unrestricted conformational transitions and a decoupled functioning of its individual enzymatic entities. With this architecture, AROM poses a tractable test case for the effects of active site proximity on the efficiency of both natural metabolic systems and biotechnological pathway optimization approaches. We show that a mere colocalization of enzymes is not sufficient to yield a detectable improvement of metabolic throughput.

Architecture and functional dynamics of the pentafunctional AROM complex.,Arora Veraszto H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD Nat Chem Biol. 2020 Jul 6. pii: 10.1038/s41589-020-0587-9. doi:, 10.1038/s41589-020-0587-9. PMID:32632294[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Arora Veraszto H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD. Architecture and functional dynamics of the pentafunctional AROM complex. Nat Chem Biol. 2020 Jul 6. pii: 10.1038/s41589-020-0587-9. doi:, 10.1038/s41589-020-0587-9. PMID:32632294 doi:http://dx.doi.org/10.1038/s41589-020-0587-9

6hqv, resolution 3.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=6hqv&oldid=3268974"