1c7n: Difference between revisions

Revision as of 12:25, 2 May 2008

CRYSTAL STRUCTURE OF CYSTALYSIN FROM TREPONEMA DENTICOLA CONTAINS A PYRIDOXAL 5'-PHOSPHATE COFACTOR

OverviewOverview

Cystalysin is a C(beta)-S(gamma) lyase from the oral pathogen Treponema denticola catabolyzing L-cysteine to produce pyruvate, ammonia and H(2)S. With its ability to induce cell lysis, cystalysin represents a new class of pyridoxal 5'-phosphate (PLP)-dependent virulence factors. The crystal structure of cystalysin was solved at 1.9 A resolution and revealed a folding and quaternary arrangement similar to aminotransferases. Based on the active site architecture, a detailed catalytic mechanism is proposed for the catabolism of S-containing amino acid substrates yielding H(2)S and cysteine persulfide. Since no homologies were observed with known haemolysins the cytotoxicity of cystalysin is attributed to this chemical reaction. Analysis of the cystalysin-L-aminoethoxyvinylglycine (AVG) complex revealed a 'dead end' ketimine PLP derivative, resulting in a total loss of enzyme activity. Cystalysin represents an essential factor of adult periodontitis, therefore the structure of the cystalysin-AVG complex may provide the chemical basis for rational drug design.

About this StructureAbout this Structure

1C7N is a Single protein structure of sequence from Treponema denticola. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme., Krupka HI, Huber R, Holt SC, Clausen T, EMBO J. 2000 Jul 3;19(13):3168-78. PMID:10880431 Page seeded by OCA on Fri May 2 12:25:38 2008

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OCA

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 [[Image:1c7n.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1c7n |SIZE=350|CAPTION= <scene name='initialview01'>1c7n</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_1c7n", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1c7n|  PDB=1c7n  |  SCENE=  }}
-|RELATEDENTRY=[[1c7o|1C7O]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c7n OCA], [http://www.ebi.ac.uk/pdbsum/1c7n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c7n RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF CYSTALYSIN FROM TREPONEMA DENTICOLA CONTAINS A PYRIDOXAL 5'-PHOSPHATE COFACTOR'''
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 [[Category: Huber, R.]]
 [[Category: Krupka, H I.]]
-[[Category: aminotransferase]]
+[[Category: Aminotransferase]]
-[[Category: pyridoxal phosphate]]
+[[Category: Pyridoxal phosphate]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:25:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:16:16 2008''