2vk2: Difference between revisions
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==Crystal structure of a galactofuranose binding protein== | ==Crystal structure of a galactofuranose binding protein== | ||
<StructureSection load='2vk2' size='340' side='right' caption='[[2vk2]], [[Resolution|resolution]] 1.20Å' scene=''> | <StructureSection load='2vk2' size='340' side='right'caption='[[2vk2]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VK2 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2vk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VK2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VK2 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GZL:BETA-D-GALACTOFURANOSE'>GZL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GZL:BETA-D-GALACTOFURANOSE'>GZL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vk2 OCA], [http://pdbe.org/2vk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/2vk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vk2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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==See Also== | ==See Also== | ||
*[[ABC transporter|ABC transporter]] | *[[ABC transporter 3D structures|ABC transporter 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Ecoli]] | [[Category: Ecoli]] | ||
[[Category: Large Structures]] | |||
[[Category: Horler, R S.P]] | [[Category: Horler, R S.P]] | ||
[[Category: Muller, A]] | [[Category: Muller, A]] | ||
Revision as of 11:02, 25 June 2020
Crystal structure of a galactofuranose binding proteinCrystal structure of a galactofuranose binding protein
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe widespread utilization of sugars by microbes is reflected in the diversity and multiplicity of cellular transporters used to acquire these compounds from the environment. The model bacterium Escherichia coli has numerous transporters that allow it to take up hexoses and pentoses, which recognize the more abundant pyranose forms of these sugars. Here we report the biochemical and structural characterization of a transporter protein YtfQ from E. coli that forms part of an uncharacterized ABC transporter system. Remarkably the crystal structure of this protein, solved to 1.2 A using x-ray crystallography, revealed that YtfQ binds a single molecule of galactofuranose in its ligand binding pocket. Selective binding of galactofuranose over galactopyranose was also observed using NMR methods that determined the form of the sugar released from the protein. The pattern of expression of the ytfQRTyjfF operon encoding this transporter mirrors that of the high affinity galactopyranose transporter of E. coli, suggesting that this bacterium has evolved complementary transporters that enable it to use all the available galactose present during carbon limiting conditions. Furanose-specific sugar transport: characterization of a bacterial galactofuranose-binding protein.,Horler RS, Muller A, Williamson DC, Potts JR, Wilson KS, Thomas GH J Biol Chem. 2009 Nov 6;284(45):31156-63. Epub 2009 Sep 10. PMID:19744923[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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