6oc6: Difference between revisions

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<StructureSection load='6oc6' size='340' side='right'caption='[[6oc6]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
<StructureSection load='6oc6' size='340' side='right'caption='[[6oc6]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6oc6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OC6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OC6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6oc6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metea Metea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OC6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OC6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xoxF, MexAM1_META1p1740 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272630 METEA])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oc6 OCA], [http://pdbe.org/6oc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oc6 RCSB], [http://www.ebi.ac.uk/pdbsum/6oc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oc6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oc6 OCA], [http://pdbe.org/6oc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oc6 RCSB], [http://www.ebi.ac.uk/pdbsum/6oc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oc6 ProSAT]</span></td></tr>
</table>
</table>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Metea]]
[[Category: Fellner, M]]
[[Category: Fellner, M]]
[[Category: Good, N M]]
[[Category: Good, N M]]

Revision as of 09:52, 25 June 2020

Lanthanide-dependent methanol dehydrogenase XoxF from Methylobacterium extorquens, in complex with Lanthanum and Pyrroloquinoline quinoneLanthanide-dependent methanol dehydrogenase XoxF from Methylobacterium extorquens, in complex with Lanthanum and Pyrroloquinoline quinone

Structural highlights

6oc6 is a 2 chain structure with sequence from Metea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:xoxF, MexAM1_META1p1740 (METEA)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The lanthanide elements (Ln(3+)), those with atomic numbers 57-63 (excluding promethium, Pm(3+)), form a cofactor complex with pyrroloquinoline quinone (PQQ) in bacterial XoxF methanol dehydrogenases (MDHs) and ExaF ethanol dehydrogenases (EDHs), expanding the range of biological elements and opening novel areas of metabolism and ecology. Other MDHs, known as MxaFIs, are related in sequence and structure to these proteins, yet they instead possess a Ca(2+)-PQQ cofactor. An important missing piece of the Ln(3+) puzzle is defining what features distinguish enzymes that use Ln(3+)-PQQ cofactors from those that do not. Here, using XoxF1 MDH from the model methylotrophic bacterium Methylorubrum extorquens AM1, we investigated the functional importance of a proposed lanthanide-coordinating aspartate residue. We report two crystal structures of XoxF1, one with and another without PQQ, both with La(3+) bound in the active-site region and coordinated by Asp(320) Using constructs to produce either recombinant XoxF1 or its D320A variant, we show that Asp(320) is needed for in vivo catalytic function, in vitro activity, and La(3+) coordination. XoxF1 and XoxF1 D320A, when produced in the absence of La(3+), coordinated Ca(2+), but exhibited little or no catalytic activity. We also generated the parallel substitution in ExaF to produce ExaF D319S and found that this variant loses the capacity for efficient ethanol oxidation with La(3+) These results provide evidence that a Ln(3+)-coordinating aspartate is essential for the enzymatic functions of XoxF MDHs and ExaF EDHs, supporting the notion that sequences of these enzymes, and the genes that encode them, are markers for Ln(3+) metabolism.

Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function.,Good NM, Fellner M, Demirer K, Hu J, Hausinger RP, Martinez-Gomez NC J Biol Chem. 2020 May 4. pii: RA120.013227. doi: 10.1074/jbc.RA120.013227. PMID:32366463[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Good NM, Fellner M, Demirer K, Hu J, Hausinger RP, Martinez-Gomez NC. Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function. J Biol Chem. 2020 May 4. pii: RA120.013227. doi: 10.1074/jbc.RA120.013227. PMID:32366463 doi:http://dx.doi.org/10.1074/jbc.RA120.013227

6oc6, resolution 2.89Å

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