2v8e: Difference between revisions
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==Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.== | ==Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.== | ||
<StructureSection load='2v8e' size='340' side='right' caption='[[2v8e]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='2v8e' size='340' side='right'caption='[[2v8e]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2v8e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8E OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2v8e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2V8E FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fhc|1fhc]], [[1hfh|1hfh]], [[2bzm|2bzm]], [[2g7i|2g7i]], [[2jgx|2jgx]], [[1haq|1haq]], [[1hcc|1hcc]], [[1hfi|1hfi]], [[1kov|1kov]], [[2jgw|2jgw]], [[2uwn|2uwn]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fhc|1fhc]], [[1hfh|1hfh]], [[2bzm|2bzm]], [[2g7i|2g7i]], [[2jgx|2jgx]], [[1haq|1haq]], [[1hcc|1hcc]], [[1hfi|1hfi]], [[1kov|1kov]], [[2jgw|2jgw]], [[2uwn|2uwn]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8e OCA], [http://pdbe.org/2v8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v8e RCSB], [http://www.ebi.ac.uk/pdbsum/2v8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v8e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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==See Also== | ==See Also== | ||
*[[Complement factor|Complement factor]] | *[[Complement factor 3D structures|Complement factor 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
[[Category: Large Structures]] | |||
[[Category: Barlow, P N]] | [[Category: Barlow, P N]] | ||
[[Category: Blaum, B S]] | [[Category: Blaum, B S]] | ||
Revision as of 14:22, 17 June 2020
Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedNearly 50 million people worldwide suffer from age-related macular degeneration (AMD), which causes severe loss of central vision. A single-nucleotide polymorphism in the gene for the complement regulator factor H (FH), which causes a Tyr-to-His substitution at position 402, is linked to approximately 50% of attributable risks for AMD. We present the crystal structure of the region of FH containing the polymorphic amino acid His402 in complex with an analogue of the glycosaminoglycans (GAGs) that localize the complement regulator on the cell surface. The structure demonstrates direct coordination of ligand by the disease-associated polymorphic residue, providing a molecular explanation of the genetic observation. This glycan-binding site occupies the center of an extended interaction groove on the regulator's surface, implying multivalent binding of sulfated GAGs. This finding is confirmed by structure-based site-directed mutagenesis, nuclear magnetic resonance-monitored binding experiments performed for both H402 and Y402 variants with this and another model GAG, and analysis of an extended GAG-FH complex. Structural basis for complement factor H linked age-related macular degeneration.,Prosser BE, Johnson S, Roversi P, Herbert AP, Blaum BS, Tyrrell J, Jowitt TA, Clark SJ, Tarelli E, Uhrin D, Barlow PN, Sim RB, Day AJ, Lea SM J Exp Med. 2007 Oct 1;204(10):2277-83. Epub 2007 Sep 24. PMID:17893204[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Human
- Large Structures
- Barlow, P N
- Blaum, B S
- Clark, S J
- Day, A J
- Herbert, A P
- Johnson, S
- Jowitt, T A
- Lea, S M
- Prosser, B E
- Roversi, P
- Sim, R B
- Tarelli, E
- Tyrrell, J
- Uhrin, D
- Age-related macular degeneration
- Alternative splicing
- Complement
- Complement alternate pathway
- Disease mutation
- Factor h
- Glycoprotein
- Glycosaminoglycan
- Immune response
- Immune system
- Innate immunity
- Polymorphism
- Secreted
- Sucrose octasulphate
- Sushi