2ro2: Difference between revisions
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==Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme== | ==Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme== | ||
<StructureSection load='2ro2' size='340' side='right' caption='[[2ro2]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | <StructureSection load='2ro2' size='340' side='right'caption='[[2ro2]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RO2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ro2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro2 OCA], [http://pdbe.org/2ro2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ro2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ro2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ro2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Dufour, D]] | [[Category: Dufour, D]] | ||
[[Category: Flores, R]] | [[Category: Flores, R]] | ||
Revision as of 10:14, 10 June 2020
Solution structure of domain I of the negative polarity CChMVd hammerhead ribozymeSolution structure of domain I of the negative polarity CChMVd hammerhead ribozyme
Structural highlights
Publication Abstract from PubMedLoop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of chrysanthemum chlorotic mottle viroid RNA. In both hammerheads, loop 1 is a heptanucleotide hairpin loop containing an exposed U at its 5' side and an extrahelical U at its 3'-side critical for the catalytic activity of the ribozyme in vitro and for viroid infectivity in vivo, whereas loop 2 has a key opened A at its 3'-side. These structural features promote a specific loop-loop interaction motif across the major groove. The essential features of this tertiary structure element, base pairing between the 5' U of loop 1 and the 3' A of loop 2, and interaction of the extrahelical pyrimidine of loop 1 with loop 2, are likely shared by a significant fraction of natural hammerheads. Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads.,Dufour D, de la Pena M, Gago S, Flores R, Gallego J Nucleic Acids Res. 2008 Nov 29. PMID:19043070[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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