2pro: Difference between revisions
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==PRO REGION OF ALPHA-LYTIC PROTEASE== | ==PRO REGION OF ALPHA-LYTIC PROTEASE== | ||
<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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==See Also== | ==See Also== | ||
*[[Alpha-lytic protease|Alpha-lytic protease]] | *[[Alpha-lytic protease 3D structures|Alpha-lytic protease 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 29487]] | [[Category: Atcc 29487]] | ||
[[Category: Large Structures]] | |||
[[Category: Agard, D A]] | [[Category: Agard, D A]] | ||
[[Category: Mau, T]] | [[Category: Mau, T]] | ||
Revision as of 09:57, 3 June 2020
PRO REGION OF ALPHA-LYTIC PROTEASEPRO REGION OF ALPHA-LYTIC PROTEASE
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWhile the majority of proteins fold rapidly and spontaneously to their native states, the extracellular bacterial protease alpha-lytic protease (alphaLP) has a t(1/2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1). AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction. Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9). In the absence of Pro, alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report the crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP. The C-shaped Pro surrounds the C-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier. Structure of alpha-lytic protease complexed with its pro region.,Sauter NK, Mau T, Rader SD, Agard DA Nat Struct Biol. 1998 Nov;5(11):945-50. PMID:9808037[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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