5h93: Difference between revisions
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==Crystal structure of Geobacter metallireducens SMUG1== | ==Crystal structure of Geobacter metallireducens SMUG1== | ||
<StructureSection load='5h93' size='340' side='right' caption='[[5h93]], [[Resolution|resolution]] 2.18Å' scene=''> | <StructureSection load='5h93' size='340' side='right'caption='[[5h93]], [[Resolution|resolution]] 2.18Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5h93]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H93 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5h93]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H93 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H93 FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h9i|5h9i]], [[5h99|5h99]], [[5h98|5h98]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h9i|5h9i]], [[5h99|5h99]], [[5h98|5h98]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h93 OCA], [http://pdbe.org/5h93 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h93 RCSB], [http://www.ebi.ac.uk/pdbsum/5h93 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h93 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Cao, W]] | [[Category: Cao, W]] | ||
[[Category: Shen, J]] | [[Category: Shen, J]] | ||
Revision as of 09:55, 20 May 2020
Crystal structure of Geobacter metallireducens SMUG1Crystal structure of Geobacter metallireducens SMUG1
Structural highlights
Publication Abstract from PubMedBase deamination is a common type of DNA damage that occurs in all organisms. DNA repair mechanisms are critical to maintain genome integrity, in which the base excision repair pathway plays an essential role. In the BER pathway, the uracil DNA glycosylase superfamily is responsible for removing the deaminated bases from DNA and generates apurinic/apyrimidinic (AP) sites. Geobacter metallireducens SMUG1 (GmeSMUG1) is an interesting family 3 enzyme in the UDG superfamily, with dual substrate specificities for DNA with uracil or xanthine. In contrast, the mutant G63P of GmeSMUG1 has exclusive activity for uracil, while N58D is inactive for both substrates, as we have reported previously. However, the structural bases for these substrate specificities are not well understood. In this study, we solved a series of crystal structures of WT and mutants of GmeSMUG1 at relatively high resolutions. These structures provide insight on the molecular mechanism of xanthine recognition for GmeSMUG1 and indicate that H210 plays a key role in xanthine recognition, which is in good agreement with the results of our EMSA and activity assays. More importantly, our mutant structures allow us to build models to rationalize our previous experimental observations of altered substrate activities of these mutants. Structural Basis of Substrate Specificity in Geobacter metallireducens SMUG1.,Zhang Z, Shen J, Yang Y, Li J, Cao W, Xie W ACS Chem Biol. 2016 Apr 22. PMID:27071000[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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