1bcu: Difference between revisions

Revision as of 11:20, 2 May 2008

ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN

OverviewOverview

Proflavin can be used to study the interactions of inhibitors and substrates with thrombin by monitoring the changes in the visible absorption spectrum that occur on dye displacement. We have used microspectrophotometric methods to investigate the binding of proflavin to crystals of an alpha-thrombin-hirugen complex and have determined the structure by X-ray crystallography. The proflavin molecule binds in the S1 pocket of the enzyme with one of the amino groups hydrogen bonded to the carboxylate of Asp-189 while the protonated ring nitrogen is hydrogen bonded to the carbonyl of Gly-219. This result indicates that the proflavin displacement assay can be used to specifically monitor the binding of inhibitors to the S1 pocket.

About this StructureAbout this Structure

1BCU is a Protein complex structure of sequences from Hirudo medicinalis and Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin., Conti E, Rivetti C, Wonacott A, Brick P, FEBS Lett. 1998 Mar 27;425(2):229-33. PMID:9559654 Page seeded by OCA on Fri May 2 11:20:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1bcu.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1bcu |SIZE=350|CAPTION= <scene name='initialview01'>1bcu</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1bcu", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene>, <scene name='pdbligand=TYS:SULFONATED+TYROSINE'>TYS</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1bcu|  PDB=1bcu  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcu OCA], [http://www.ebi.ac.uk/pdbsum/1bcu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bcu RCSB]</span>
-}}
 '''ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN'''
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 [[Category: Rivetti, C.]]
 [[Category: Wonacott, A.]]
-[[Category: complex (serine protease inhibitor)]]
+[[Category: Hydrolase]]
-[[Category: hydrolase]]
+[[Category: Serine protease]]
-[[Category: serine protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:20:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:58:19 2008''