6q22: Difference between revisions
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==Coiled-coil Trimer with Glu:Aminobutyric acid:Lys Triad== | ==Coiled-coil Trimer with Glu:Aminobutyric acid:Lys Triad== | ||
<StructureSection load='6q22' size='340' side='right'caption='[[6q22]]' scene=''> | <StructureSection load='6q22' size='340' side='right'caption='[[6q22]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Q22 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6q22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Q22 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6q22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q22 OCA], [http://pdbe.org/6q22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q22 RCSB], [http://www.ebi.ac.uk/pdbsum/6q22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q22 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6q22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q22 OCA], [http://pdbe.org/6q22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q22 RCSB], [http://www.ebi.ac.uk/pdbsum/6q22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q22 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Here we show that a solvent-exposed f-position (i.e., residue 14) within a well-characterized trimeric helix bundle can facilitate a stabilizing long-range synergistic interaction involving b-position Glu10 (i.e., i - 4 relative to residue 14) and c-position Lys18 (i.e., i + 4), depending the identity of residue 14. The extent of stabilization associated with the Glu10-Lys18 pair depends primarily on the presence of a side-chain hydrogen-bond donor at residue 14; the nonpolar or hydrophobic character of residue 14 plays a smaller but still significant role. Crystal structures and molecular dynamics simulations indicate that Glu10 and Lys18 do not interact directly with each other but suggest the possibility that the proximity of residue 14 with Lys18 allows Glu10 to interact favorably with nearby Lys7. Subsequent thermodynamic experiments confirm the important role of Lys7 in the large synergistic stabilization associated with the Glu10-Lys18 pair. Our results highlight the exquisite complexity and surprising long-range synergistic interactions among b-, c-, and f-position residues within helix bundles, suggesting new possibilities for engineering hyperstable helix bundles and emphasizing the need to consider carefully the impact of substitutions at these positions for application-specific purposes. | |||
Context-Dependent Stabilizing Interactions among Solvent-Exposed Residues along the Surface of a Trimeric Helix Bundle.,Stern KL, Smith MS, Billings WM, Loftus TJ, Conover BM, Della Corte D, Price JL Biochemistry. 2020 Apr 20. doi: 10.1021/acs.biochem.0c00045. PMID:32270676<ref>PMID:32270676</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6q22" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Billings | [[Category: Billings, W M]] | ||
[[Category: Price | [[Category: Price, J L]] | ||
[[Category: Smith | [[Category: Smith, M S]] | ||
[[Category: Stern | [[Category: Stern, K L]] | ||
[[Category: De novo protein]] | |||
[[Category: Helix]] | |||
[[Category: Trimer]] | |||