1bcm: Difference between revisions
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'''BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT''' | '''BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT''' | ||
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[[Category: Mizuuchi, K.]] | [[Category: Mizuuchi, K.]] | ||
[[Category: Rice, P A.]] | [[Category: Rice, P A.]] | ||
[[Category: | [[Category: Dna binding]] | ||
[[Category: | [[Category: Endonuclease]] | ||
[[Category: | [[Category: Integrase]] | ||
[[Category: | [[Category: Polynucleotidyl transferase]] | ||
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Revision as of 11:20, 2 May 2008
BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT
OverviewOverview
The crystal structure of the core domain of bacteriophage Mu transposase, MuA, has been determined at 2.4 A resolution. The first of two subdomains contains the active site and, despite very limited sequence homology, exhibits a striking similarity to the core domain of HIV-1 integrase, which carries out a similar set of biochemical reactions. It also exhibits more limited similarity to other nucleases, RNase H and RuvC. The second, a beta barrel, connects to the first subdomain through several contacts. Three independent determinations of the monomer structure from two crystal forms all show the active site held in a similar, apparently inactive configuration. The enzymatic activity of MuA is known to be activated by formation of a DNA-bound tetramer of the protein. We propose that the connections between the two subdomains may be involved in the cross-talk between the active site and the other domains of the transposase that controls the activity of the protein.
About this StructureAbout this Structure
1BCM is a Single protein structure of sequence from Enterobacteria phage mu. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration., Rice P, Mizuuchi K, Cell. 1995 Jul 28;82(2):209-20. PMID:7628012 Page seeded by OCA on Fri May 2 11:20:37 2008