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==6-phospho-beta-glucosidase== | ==6-phospho-beta-glucosidase== | ||
<StructureSection load='5foo' size='340' side='right' caption='[[5foo]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='5foo' size='340' side='right'caption='[[5foo]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5foo]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOO OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5foo]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FOO FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=JAZ:6-PHOSPHOCYCLOPHELLITOL'>JAZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=JAZ:6-PHOSPHOCYCLOPHELLITOL'>JAZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5foo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5foo OCA], [http://pdbe.org/5foo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5foo RCSB], [http://www.ebi.ac.uk/pdbsum/5foo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5foo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 16: | Line 16: | ||
</div> | </div> | ||
<div class="pdbe-citations 5foo" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5foo" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Davies, G J]] | [[Category: Davies, G J]] | ||
[[Category: Jin, Y]] | [[Category: Jin, Y]] | ||
Revision as of 10:29, 6 May 2020
6-phospho-beta-glucosidase6-phospho-beta-glucosidase
Structural highlights
Publication Abstract from PubMedCovalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-beta-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation. Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases.,Kwan DH, Jin Y, Jiang J, Chen HM, Kotzler MP, Overkleeft HS, Davies GJ, Withers SG FEBS Lett. 2016 Feb;590(4):461-8. doi: 10.1002/1873-3468.12059. Epub 2016 Feb 14. PMID:26790390[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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