5fid: Difference between revisions
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==Crystal structure of the protein elicitor MoHrip2 from Magnaporthe oryzae== | ==Crystal structure of the protein elicitor MoHrip2 from Magnaporthe oryzae== | ||
<StructureSection load='5fid' size='340' side='right' caption='[[5fid]], [[Resolution|resolution]] 1.81Å' scene=''> | <StructureSection load='5fid' size='340' side='right'caption='[[5fid]], [[Resolution|resolution]] 1.81Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5fid]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FID OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5fid]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpi_841383_[[magnaporthe_oryzae]] Bpi 841383 [[magnaporthe oryzae]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FID OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FID FirstGlance]. <br> | ||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hrip2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=318829 BPI 841383 [[Magnaporthe oryzae]]])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fid OCA], [http://pdbe.org/5fid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fid RCSB], [http://www.ebi.ac.uk/pdbsum/5fid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fid ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Duan, L]] | [[Category: Duan, L]] | ||
[[Category: Liu, M]] | [[Category: Liu, M]] | ||
Revision as of 10:20, 6 May 2020
Crystal structure of the protein elicitor MoHrip2 from Magnaporthe oryzaeCrystal structure of the protein elicitor MoHrip2 from Magnaporthe oryzae
Structural highlights
Publication Abstract from PubMedThe protein elicitor MoHrip2, which was extracted from Magnaporthe oryzae as an exocrine protein, triggers the tobacco immune system and enhances blast resistance in rice. However, the detailed mechanisms by which MoHrip2 acts as an elicitor remain unclear. Here, we investigated the structure of MoHrip2 to elucidate its functions based on molecular structure. The three-dimensional structure of MoHrip2 was obtained. Overall, the crystal structure formed a beta-barrel structure and showed high similarity to the pathogenesis-related (PR) thaumatin superfamily protein thaumatin-like xylanase inhibitor (TL-XI). To investigate the functional regions responsible for MoHrip2 elicitor activities, the full length and eight truncated proteins were expressed in Escherichia coli and were evaluated for elicitor activity in tobacco. Biological function analysis showed that MoHrip2 triggered the defense system against Botrytis cinerea in tobacco. Moreover, only MoHrip2M14 and other fragments containing the 14 amino acids residues in the middle region of the protein showed the elicitor activity of inducing a hypersensitive response and resistance related pathways, which were similar to that of full-length MoHrip2. These results revealed that the central 14 amino acid residues were essential for anti-pathogenic activity. Crystal Structure Analysis and the Identification of Distinctive Functional Regions of the Protein Elicitor Mohrip2.,Liu M, Duan L, Wang M, Zeng H, Liu X, Qiu D Front Plant Sci. 2016 Jul 26;7:1103. doi: 10.3389/fpls.2016.01103. eCollection, 2016. PMID:27507984[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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