6l86: Difference between revisions

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<StructureSection load='6l86' size='340' side='right'caption='[[6l86]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
<StructureSection load='6l86' size='340' side='right'caption='[[6l86]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6l86]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L86 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6l86]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_thioluteus"_okami_1952 "streptomyces thioluteus" okami 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L86 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L86 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l86 OCA], [http://pdbe.org/6l86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l86 RCSB], [http://www.ebi.ac.uk/pdbsum/6l86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l86 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l86 OCA], [http://pdbe.org/6l86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l86 RCSB], [http://www.ebi.ac.uk/pdbsum/6l86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l86 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Streptomyces thioluteus okami 1952]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chang, W]]
[[Category: Chang, W]]

Revision as of 09:26, 6 May 2020

The structure of SfaAThe structure of SfaA

Structural highlights

6l86 is a 4 chain structure with sequence from "streptomyces_thioluteus"_okami_1952 "streptomyces thioluteus" okami 1952. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

N-alkylisonitrile, a precursor to isonitrile-containing lipopeptides, is biosynthesized via decarboxylation-assisted -N identical withC group (isonitrile) formation by using N-alkylglycine as the substrate. This reaction is catalyzed by iron(II) and 2-oxoglutarate (Fe/2OG) dependent enzymes. Distinct from typical oxygenation or halogenation reactions catalyzed by this class of enzymes, installation of the isonitrile group represents a novel reaction type for Fe/2OG enzymes that involves a four-electron oxidative process. Here we report the plausible mechanism of three Fe/2OG enzymes, Sav607, ScoE and SfaA, catalyzed isonitrile formation. The X-ray structures of iron loaded ScoE in complex with its substrate and the intermediate, along with biochemical and biophysical data reveal that -N identical withC bond formation involves two cycles of Fe/2OG enzyme catalysis. The reaction starts with an Fe(IV)-oxo catalyzed hydroxylation. It is likely followed by decarboxylation-assisted desaturation to complete isonitrile installation.

Pathway from N-alkylglycine to alkylisonitrile catalyzed by iron(II) and 2-oxoglutarate dependent oxygenases.,Chang WC, Chen TY, Chen J, Tang Y, Zhou J, Guo Y Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.201914896. PMID:32074393[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Chang WC, Chen TY, Chen J, Tang Y, Zhou J, Guo Y. Pathway from N-alkylglycine to alkylisonitrile catalyzed by iron(II) and 2-oxoglutarate dependent oxygenases. Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.201914896. PMID:32074393 doi:http://dx.doi.org/10.1002/anie.201914896

6l86, resolution 2.23Å

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