1b8j: Difference between revisions

Revision as of 11:12, 2 May 2008

ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE

OverviewOverview

A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.

About this StructureAbout this Structure

1B8J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061 Page seeded by OCA on Fri May 2 11:12:15 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1b8j.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1b8j |SIZE=350|CAPTION= <scene name='initialview01'>1b8j</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_1b8j", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SVA:SERINE+VANADATE'>SVA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1b8j|  PDB=1b8j  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8j OCA], [http://www.ebi.ac.uk/pdbsum/1b8j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b8j RCSB]</span>
-}}
 '''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE'''
@@ Line 29: / Line 26: @@
 [[Category: Kantrowitz, E R.]]
 [[Category: Stec, B.]]
-[[Category: alcohol acceptor)]]
+[[Category: Alkaline phosphatase]]
-[[Category: alkaline phosphatase]]
+[[Category: Hydrolase]]
-[[Category: hydrolase]]
+[[Category: Phosphoric monoester]]
-[[Category: phosphoric monoester]]
+[[Category: Vanadate]]
-[[Category: transferase (phospho]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:12:15 2008''
-[[Category: vanadate]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:55:46 2008''