5f44: Difference between revisions
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==Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG== | ==Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG== | ||
<StructureSection load='5f44' size='340' side='right' caption='[[5f44]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='5f44' size='340' side='right'caption='[[5f44]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5f44]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F44 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5f44]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacrg Lacrg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F44 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F44 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5faa|5faa]], [[5fgs|5fgs]], [[5fgr|5fgr]], [[5fie|5fie]], [[5hbb|5hbb]], [[5hdl|5hdl]], [[5hts|5hts]], [[5j4m|5j4m]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5faa|5faa]], [[5fgs|5fgs]], [[5fgr|5fgr]], [[5fie|5fie]], [[5hbb|5hbb]], [[5hdl|5hdl]], [[5hts|5hts]], [[5j4m|5j4m]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LRHM_0426 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f44 OCA], [http://pdbe.org/5f44 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f44 RCSB], [http://www.ebi.ac.uk/pdbsum/5f44 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f44 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Lacrg]] | |||
[[Category: Large Structures]] | |||
[[Category: Chaurasia, P]] | [[Category: Chaurasia, P]] | ||
[[Category: Krishnan, V]] | [[Category: Krishnan, V]] | ||
Revision as of 10:58, 29 April 2020
Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GGCrystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG
Structural highlights
Publication Abstract from PubMedThus far, all solved structures of pilin-proteins comprising sortase-assembled pili are from pathogenic genera and species. Here, we present the first crystal structure of a pilin subunit (SpaA) from a non-pathogen host (Lactobacillus rhamnosus GG). SpaA consists of two tandem CnaB-type domains, each with an isopeptide bond and E-box motif. Intriguingly, while the isopeptide bond in the N-terminal domain forms between lysine and asparagine, the one in the C-terminal domain atypically involves aspartate. We also solved crystal structures of mutant proteins where residues implicated in forming isopeptide bonds were replaced. Expectedly, the E-box-substituted E139A mutant lacks an isopeptide bond in the N-terminal domain. However, the C-terminal E269A substitution gave two structures; one of both domains with their isopeptide bonds present, and another of only the N-terminal domain, but with an unformed isopeptide bond and significant conformational changes. This latter crystal structure has never been observed for any other Gram-positive pilin. Notably, the C-terminal isopeptide bond still forms in D295N-substituted SpaA, irrespective of E269 being present or absent. Although E-box mutations affect SpaA proteolytic and thermal stability, a cumulative effect perturbing normal pilus polymerization was unobserved. A model showing the polymerized arrangement of SpaA within the SpaCBA pilus is proposed. New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit.,Chaurasia P, Pratap S, von Ossowski I, Palva A, Krishnan V Sci Rep. 2016 Jun 28;6:28664. doi: 10.1038/srep28664. PMID:27349405[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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