6lvu: Difference between revisions
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==Crystal structure of apo acyl carrier protein from Thermotoga maritima== | ==Crystal structure of apo acyl carrier protein from Thermotoga maritima== | ||
<StructureSection load='6lvu' size='340' side='right'caption='[[6lvu]]' scene=''> | <StructureSection load='6lvu' size='340' side='right'caption='[[6lvu]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LVU FirstGlance]. <br> | <table><tr><td colspan='2'>[[6lvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LVU FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lvu OCA], [http://pdbe.org/6lvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lvu RCSB], [http://www.ebi.ac.uk/pdbsum/6lvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lvu ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lvu OCA], [http://pdbe.org/6lvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lvu RCSB], [http://www.ebi.ac.uk/pdbsum/6lvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lvu ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/ACP_THEMA ACP_THEMA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Thermotoga maritima, a deep-branching hyperthermophilic bacterium, expresses an extraordinarily stable Thermotoga maritima acyl carrier protein (Tm-ACP) that functions as a carrier in the fatty acid synthesis system at near-boiling aqueous environments. Here, to understand the hyperthermal adaptation of Tm-ACP, we investigated the structure and dynamics of Tm-ACP by nuclear magnetic resonance (NMR) spectroscopy. The melting temperature of Tm-ACP (101.4 degrees C) far exceeds that of other ACPs, owing to extensive ionic interactions and tight hydrophobic packing. The D59 residue, which replaces Pro/Ser of other ACPs, mediates ionic clustering between helices III and IV. This creates a wide pocket entrance to facilitate the accommodation of long acyl chains required for hyperthermal adaptation of the T. maritima cell membrane. Tm-ACP is revealed to be the first ACP that harbor an amide proton hyperprotected against hydrogen/deuterium exchange for I15. The hydrophobic interactions mediated by I15 appear to be the key driving forces of the global folding process of Tm-ACP. Our findings provide insights into the structural basis of the hyperthermal adaptation of ACP, which might have allowed T. maritima to survive in hot ancient oceans. | |||
Structural Characterization of an ACP from Thermotoga maritima: Insights into Hyperthermal Adaptation.,Lee Y, Jang A, Jeong MC, Park N, Park J, Lee WC, Cheong C, Kim Y Int J Mol Sci. 2020 Apr 9;21(7). pii: ijms21072600. doi: 10.3390/ijms21072600. PMID:32283632<ref>PMID:32283632</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6lvu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kim Y]] | [[Category: Thema]] | ||
[[Category: Lee | [[Category: Kim, Y]] | ||
[[Category: Lee Y]] | [[Category: Lee, W C]] | ||
[[Category: Lee, Y]] | |||
[[Category: Acyl carrier protein]] | |||
[[Category: Biosynthetic protein]] | |||