Sandbox Reserved 1605: Difference between revisions
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== Relevance == | == Relevance == | ||
The cytochrome ''bd'' oxidase is essential for bacteria to thrive in the human body. Any alteration of the bacterial Cyd subunits produces a nonfunctional mutant cytochrome ''bd'' oxidase<ref name="Moosa">PMID: 28760899</ref>, which inhibits bacterial growth. Bacterial terminal oxidases are essential for formate oxidation activity, which enhances bacterial growth. If ''E. coli'' were missing or possessed ineffective CydA and B subunits, their growth advantage was eliminated<ref name="Hughes">PMID: 28182951</ref>. With [https://en.wikipedia.org/wiki/Colitis colitis], ''E. coli'' mutants that were missing CydAB colonized poorly, in comparison to the wild type levels of colonization<ref name="Hughes">PMID: 28182951</ref>. The cytochrome ''bd'' oxidase is the main component in nitric oxide (NO) tolerance in bacteria, which is released by neutrophils and macrophages when the host is infected<ref name="Shepherd">PMID: 27767067</ref>. ''E. coli'' growth seen in urinary tract infections is mainly due to the NO resistant bd oxidase. Without the CydA and CydB subunits, bacteria could not colonize in high NO conditions<ref name="Shepherd">PMID: 27767067</ref>. Cytochrome ''bd'' oxidases are essential in other pathogenic bacteria such as [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis ''M. tuberculosis'']. Deletion of the CydA and CydB subunits dramatically decreased the growth of ''M. tb'' when exposed to imidazo[1,2-α]pyridine, a known inhibitor of ATP synthase<ref name="Arora">PMID:25155596</ref>. | The cytochrome ''bd'' oxidase is essential for bacteria to thrive in the human body. Any alteration of the bacterial Cyd subunits produces a nonfunctional mutant cytochrome ''bd'' oxidase<ref name="Moosa">PMID: 28760899</ref>, which inhibits bacterial growth. Bacterial terminal oxidases are essential for formate oxidation activity, which enhances bacterial growth. If ''E. coli'' were missing or possessed ineffective CydA and B subunits, their growth advantage was eliminated<ref name="Hughes">PMID: 28182951</ref>. With [https://en.wikipedia.org/wiki/Colitis colitis], ''E. coli'' mutants that were missing CydAB colonized poorly, in comparison to the wild type levels of colonization<ref name="Hughes">PMID: 28182951</ref>. The cytochrome ''bd'' oxidase is the main component in nitric oxide (NO) tolerance in bacteria, which is released by neutrophils and macrophages when the host is infected<ref name="Shepherd">PMID: 27767067</ref>. ''E. coli'' growth seen in urinary tract infections is mainly due to the NO resistant bd oxidase. Without the CydA and CydB subunits, bacteria could not colonize in high NO conditions<ref name="Shepherd">PMID: 27767067</ref>. Cytochrome ''bd'' oxidases are essential in other pathogenic bacteria such as [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis ''M. tuberculosis'']. Deletion of the CydA and CydB subunits dramatically decreased the growth of ''M. tb'' when exposed to imidazo[1,2-α]pyridine, a known inhibitor of ATP synthase<ref name="Arora">PMID:25155596</ref>. | ||
Since cytochrome ''bd'' oxidases are only found in prokaryotes and are required for pathogenic bacterial infections, inhibitors that target cytochrome ''bd'' oxidase are promising antibacterial agents. Compounds that target heme b<sub>558</sub><ref name="Harikishore">PMID: 31939065</ref>, create unusable forms of oxygen<ref name="Galván">PMID: 30790617</ref>, and target the o-channel <ref name="Lu">PMID: 26015371 </ref> have shown | |||
Since cytochrome ''bd'' oxidases are only found in prokaryotes and are required for pathogenic bacterial infections, inhibitors that target cytochrome ''bd'' oxidase are promising antibacterial agents. Compounds that target heme b<sub>558</sub><ref name="Harikishore">PMID: 31939065</ref>, create unusable forms of oxygen<ref name="Galván">PMID: 30790617</ref>, and target the o-channel <ref name="Lu">PMID: 26015371 </ref> have shown potential in halting bacterial growth. | |||