2j9d: Difference between revisions
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==Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake== | ==Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake== | ||
<StructureSection load='2j9d' size='340' side='right' caption='[[2j9d]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2j9d' size='340' side='right'caption='[[2j9d]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2j9d]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9D OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2j9d]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2J9D FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j9c|2j9c]], [[2j9e|2j9e]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j9c|2j9c]], [[2j9e|2j9e]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2j9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9d OCA], [http://pdbe.org/2j9d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j9d RCSB], [http://www.ebi.ac.uk/pdbsum/2j9d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 43067]] | [[Category: Atcc 43067]] | ||
[[Category: Large Structures]] | |||
[[Category: Kalthoff, C]] | [[Category: Kalthoff, C]] | ||
[[Category: Kuehlbrandt, W]] | [[Category: Kuehlbrandt, W]] | ||
Revision as of 10:44, 15 April 2020
Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptakeStructure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedA binary complex of the ammonia channel Amt1 from Methanococcus jannaschii and its cognate P(II) signalling protein GlnK1 has been produced and characterized. Complex formation is prevented specifically by the effector molecules Mg-ATP and 2-ketoglutarate. Single-particle electron microscopy of the complex shows that GlnK1 binds on the cytoplasmic side of Amt1. Three high-resolution X-ray structures of GlnK1 indicate that the functionally important T-loop has an extended, flexible conformation in the absence of Mg-ATP, but assumes a compact, tightly folded conformation upon Mg-ATP binding, which in turn creates a 2-ketoglutarate-binding site. We propose a regulatory mechanism by which nitrogen uptake is controlled by the binding of both effector molecules to GlnK1. At normal effector levels, a 2-ketoglutarate molecule binding at the apex of the compact T-loop would prevent complex formation, ensuring uninhibited ammonia uptake. At low levels of Mg-ATP, the extended loops would seal the ammonia channels in the complex. Binding of both effector molecules to P(II) signalling proteins may thus represent an effective feedback mechanism for regulating ammonium uptake through the membrane. Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake.,Yildiz O, Kalthoff C, Raunser S, Kuhlbrandt W EMBO J. 2007 Jan 24;26(2):589-99. Epub 2007 Jan 4. PMID:17203075[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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