6v1i

Cryo-EM reconstruction of the thermophilic bacteriophage P74-26 small terminase- symmetricCryo-EM reconstruction of the thermophilic bacteriophage P74-26 small terminase- symmetric

6v1i, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Structural highlights 6v1i is a 9 chain structure with sequence from Thermus phage p74-26. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: P74p83 (Thermus phage P74-26) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Tailed bacteriophages use a DNA-packaging motor to encapsulate their genome during viral particle assembly. The small terminase (TerS) component of this DNA-packaging machinery acts as a molecular matchmaker that recognizes both the viral genome and the main motor component, the large terminase (TerL). However, how TerS binds DNA and the TerL protein remains unclear. Here, we identified gp83 of the thermophilic bacteriophage P74-26 as the TerS protein. We found that TerS(P76-26) oligomerizes into a nonamer that binds DNA, stimulates TerL ATPase activity, and inhibits TerL nuclease activity. A cryo-EM structure of TerS(P76-26) revealed that it forms a ring with a wide central pore and radially arrayed helix-turn-helix (HTH) domains. The structure further showed that these HTH domains, which are thought to bind DNA by wrapping the double helix around the ring, are rigidly held in an orientation distinct from that seen in other TerS proteins. This rigid arrangement of the putative DNA-binding domain imposed strong constraints on how TerS(P76-26) can bind DNA. Finally, the TerS(P76-26) structure lacked the conserved C-terminal beta-barrel domain used by other TerS proteins for binding TerL. This suggests that a well-ordered C-terminal beta-barrel domain is not required for TerS(P76-26) to carry out its matchmaking function. Our work highlights a thermophilic system for studying the role of small terminase proteins in viral maturation and presents the structure of TerS(P76-26), revealing key differences between this thermophilic phage and its mesophilic counterparts. A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry.,Hayes JA, Hilbert BJ, Gaubitz C, Stone NP, Kelch BA J Biol Chem. 2020 Feb 3. pii: RA119.012224. doi: 10.1074/jbc.RA119.012224. PMID:32014998 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Terminase