6pe2

Drosophila P element transposase strand transfer complexDrosophila P element transposase strand transfer complex

6pe2, resolution 4.00Å Show:Asymmetric Unit Biological Assembly Structural highlights 6pe2 is a 10 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 6p5a Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PELET_DROME] P-element transposase that specifically mediates transposition of P-elements. Mediates both; precise and imprecise excision. Publication Abstract from PubMed P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long staggered DNA breaks during transposition. To gain insights into these features, we determined the atomic structure of the Drosophila P element transposase strand transfer complex using cryo-EM. The structure of this post-transposition nucleoprotein complex reveals that the terminal single-stranded transposon DNA adopts unusual A-form and distorted B-form helical geometries that are stabilized by extensive protein-DNA interactions. Additionally, we infer that the bound guanosine triphosphate cofactor interacts with the terminal base of the transposon DNA, apparently to position the P element DNA for catalysis. Our structure provides the first view of the P element transposase superfamily, offers new insights into P element transposition and implies a transposition pathway fundamentally distinct from other cut-and-paste DNA transposases. Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.,Ghanim GE, Kellogg EH, Nogales E, Rio DC Nat Struct Mol Biol. 2019 Oct 28. pii: 10.1038/s41594-019-0319-6. doi:, 10.1038/s41594-019-0319-6. PMID:31659330 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transposase 3D structures