1b0k: Difference between revisions

Revision as of 10:55, 2 May 2008

S642A:FLUOROCITRATE COMPLEX OF ACONITASE

OverviewOverview

The crystal structure of the S642A mutant of mitochondrial aconitase (mAc) with citrate bound has been determined at 1.8 A resolution and 100 K to capture this binding mode of substrates to the native enzyme. The 2.0 A resolution, 100 K crystal structure of the S642A mutant with isocitrate binding provides a control, showing that the Ser --> Ala replacement does not alter the binding of substrates in the active site. The aconitase mechanism requires that the intermediate product, cis-aconitate, flip over by 180 degrees about the C alpha-C beta double bond. Only one of these two alternative modes of binding, that of the isocitrate mode, has been previously visualized. Now, however, the structure revealing the citrate mode of binding provides direct support for the proposed enzyme mechanism.

About this StructureAbout this Structure

1B0K is a Single protein structure of sequence from Sus scrofa. This structure supersedes the now removed PDB entry 1as9. Full crystallographic information is available from OCA.

ReferenceReference

The mechanism of aconitase: 1.8 A resolution crystal structure of the S642a:citrate complex., Lloyd SJ, Lauble H, Prasad GS, Stout CD, Protein Sci. 1999 Dec;8(12):2655-62. PMID:10631981 Page seeded by OCA on Fri May 2 10:55:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1b0k.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1b0k", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aconitate_hydratase Aconitate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.3 4.2.1.3] </span>
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-}}
 '''S642A:FLUOROCITRATE COMPLEX OF ACONITASE'''
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 ==About this Structure==
-B0K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry 1AS9. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0K OCA].
+B0K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1as9 1as9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0K OCA].
 ==Reference==
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 [[Category: Prasad, G S.]]
 [[Category: Stout, C D.]]
-[[Category: iron-sulfur]]
+[[Category: Iron-sulfur]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: mitochondrion]]
+[[Category: Mitochondrion]]
-[[Category: transit peptide]]
+[[Category: Transit peptide]]
-[[Category: tricarboxylic acid cycle]]
+[[Category: Tricarboxylic acid cycle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:55:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:51:17 2008''