6ny2

CasX-gRNA-DNA(45bp) state ICasX-gRNA-DNA(45bp) state I

6ny2, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Structural highlights 6ny2 is a 4 chain structure with sequence from Deltaproteobacteria bacterium. This structure supersedes the now removed PDB entry 6e79. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: DD725_10130 (Deltaproteobacteria bacterium) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a. CasX enzymes comprise a distinct family of RNA-guided genome editors.,Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA Nature. 2019 Feb;566(7743):218-223. doi: 10.1038/s41586-019-0908-x. Epub 2019 Feb, 4. PMID:30718774 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.