6d73

Cryo-EM structure of the zebrafish TRPM2 channel in the presence of Ca2+Cryo-EM structure of the zebrafish TRPM2 channel in the presence of Ca2+

6d73, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Structural highlights 6d73 is a 4 chain structure with sequence from Brachidanio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Gene: trpm2 (Brachidanio rerio) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The transient receptor potential melastatin 2 (TRPM2) channel plays a key role in redox sensation in many cell types. Channel activation requires binding of both ADP-ribose (ADPR) and Ca(2+). The recently published TRPM2 structures from Danio rerio in the ligand-free and the ADPR/Ca(2+)-bound conditions represent the channel in closed and open states, which uncovered substantial tertiary and quaternary conformational rearrangements. However, it is unclear how these rearrangements are achieved within the tetrameric channel during channel gating. Here we report the cryo-electron microscopy structures of Danio rerio TRPM2 in the absence of ligands, in complex with Ca(2+) alone, and with both ADPR and Ca(2+), resolved to ~4.3 A, ~3.8 A, and ~4.2 A, respectively. In contrast to the published results, our studies capture ligand-bound TRPM2 structures in two-fold symmetric intermediate states, offering a glimpse of the structural transitions that bridge the closed and open conformations. Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel.,Yin Y, Wu M, Hsu AL, Borschel WF, Borgnia MJ, Lander GC, Lee SY Nat Commun. 2019 Aug 20;10(1):3740. doi: 10.1038/s41467-019-11733-5. PMID:31431622 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.