6jqb: Difference between revisions

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-'''Unreleased structure'''
-The entry 6jqb is ON HOLD
+==The structure of maltooligosaccharide-forming amylase from Pseudomonas saccharophila STB07 with pseudo-maltoheptaose==
+<StructureSection load='6jqb' size='340' side='right'caption='[[6jqb]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6jqb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JQB FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7SA:ACARBOSE+DERIVED+HEPTASACCHARIDE'>7SA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jqb OCA], [http://pdbe.org/6jqb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jqb RCSB], [http://www.ebi.ac.uk/pdbsum/6jqb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jqb ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The maltooligosaccharide-forming amylases (MFAses) degrade starch into maltooligosaccharides which potentially benefit human diet and grow popular in food processing, but little has been studied about their product specificity and structures. We focused on this topic and provide evidence through an X-ray crystal structure of the maltotetraose (G4)-forming amylase from Pseudomonas saccharophila STB07 (MFAps), as well as co-crystal structures of MFAps with G4 and with pseudo-maltoheptaose (pseudo-G7) determined at up to 1.1 A resolution. G4 and pseudo-G7 occupy active cleft subsites -4 to -1 and -4 to +3 respectively. Binding induces conformational changes in the active sites except Asp193, working as the base catalyst. Comparison of the MFAps structure with those of other alpha-amylases revealed obvious differences in the loop structures providing dominant interactions between protein and substrate in the non-reducing side of the active sites cleft. These structures at the non-reducing end may govern the G4 specificity of MFAps and also be relevant to its exo-type action pattern.
-Authors: Li, Z.F., Ban, X.F., Zhang, Z.Q., Li, C.M., Gu, Z.B., Jin, T.C., Li, Y.L., Shang, Y.H.
+Structure of maltotetraose-forming amylase from Pseudomonas saccharophila STB07 provides insights into its product specificity.,Zhang Z, Jin T, Xie X, Ban X, Li C, Hong Y, Cheng L, Gu Z, Li Z Int J Biol Macromol. 2019 Nov 18. pii: S0141-8130(19)37071-0. doi:, 10.1016/j.ijbiomac.2019.11.006. PMID:31751711<ref>PMID:31751711</ref>
-Description: The structure of maltooligosaccharide-forming amylase from Pseudomonas saccharophila STB07 with pseudo-maltoheptaose
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Shang, Y.H]]
+<div class="pdbe-citations 6jqb" style="background-color:#fffaf0;"></div>
-[[Category: Gu, Z.B]]
+== References ==
-[[Category: Ban, X.F]]
+<references/>
-[[Category: Li, C.M]]
+__TOC__
-[[Category: Zhang, Z.Q]]
+</StructureSection>
-[[Category: Li, Z.F]]
+[[Category: Glucan 1,4-alpha-maltotetraohydrolase]]
-[[Category: Li, Y.L]]
+[[Category: Large Structures]]
-[[Category: Jin, T.C]]
+[[Category: Ban, X F]]
+[[Category: Gu, Z B]]
+[[Category: Jin, T C]]
+[[Category: Li, C M]]
+[[Category: Li, Y L]]
+[[Category: Li, Z F]]
+[[Category: Shang, Y H]]
+[[Category: Zhang, Z Q]]
+[[Category: Hydrolase]]
+[[Category: Maltooligosaccharide-forming amylase]]
+[[Category: Saccharophila stb07]]
+[[Category: Sugar binding protein]]