5cff: Difference between revisions
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==Crystal structure of Miranda/Staufen dsRBD5 complex== | ==Crystal structure of Miranda/Staufen dsRBD5 complex== | ||
<StructureSection load='5cff' size='340' side='right' caption='[[5cff]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='5cff' size='340' side='right'caption='[[5cff]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5cff]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CFF FirstGlance]. <br> | <table><tr><td colspan='2'>[[5cff]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CFF FirstGlance]. <br> | ||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cff OCA], [http://pdbe.org/5cff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cff RCSB], [http://www.ebi.ac.uk/pdbsum/5cff PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mira, CG12249, Dmel_CG12249 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), stau, CG5753 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cff OCA], [http://pdbe.org/5cff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cff RCSB], [http://www.ebi.ac.uk/pdbsum/5cff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cff ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Drome]] | |||
[[Category: Large Structures]] | |||
[[Category: Shan, Z]] | [[Category: Shan, Z]] | ||
[[Category: Wen, W]] | [[Category: Wen, W]] | ||
[[Category: Coiled-coil and dsrna-binding domain complex]] | [[Category: Coiled-coil and dsrna-binding domain complex]] | ||
[[Category: Transcription-rna binding protein complex]] | [[Category: Transcription-rna binding protein complex]] | ||
Revision as of 14:36, 27 March 2020
Crystal structure of Miranda/Staufen dsRBD5 complexCrystal structure of Miranda/Staufen dsRBD5 complex
Structural highlights
Function[STAU_DROME] Required both for the localization of maternal determinants to the posterior pole of the egg, oskar (osk) RNA, and for correct localization to the anterior pole, anchoring bicoid (bcd) RNA. Osk protein is required to keep osk RNA and stau protein at the posterior pole. Stau-bcd complexes form particles that show a microtubule-dependent localization.[1] [2] [3] Publication Abstract from PubMedDuring the asymmetric division of Drosophila neuroblasts (NBs), the scaffold Miranda (Mira) coordinates the subcellular distribution of cell-fate determinants including Staufen (Stau) and segregates them into the ganglion mother cells (GMCs). Here we show the fifth double-stranded RNA (dsRNA)-binding domain (dsRBD5) of Stau is necessary and sufficient for binding to a coiled-coil region of Mira cargo-binding domain (CBD). The crystal structure of Mira514-595/Stau dsRBD5 complex illustrates that Mira forms an elongated parallel coiled-coil dimer, and two dsRBD5 symmetrically bind to the Mira dimer through their exposed beta-sheet faces, revealing a previously unrecognized protein interaction mode for dsRBDs. We further demonstrate that the Mira-Stau dsRBD5 interaction is responsible for the asymmetric localization of Stau during Drosophila NB asymmetric divisions. Finally, we find the CBD-mediated dimer assembly is likely a common requirement for Mira to recognize and translocate other cargos including brain tumour (Brat). The structural basis of Miranda-mediated Staufen localization during Drosophila neuroblast asymmetric division.,Jia M, Shan Z, Yang Y, Liu C, Li J, Luo ZG, Zhang M, Cai Y, Wen W, Wang W Nat Commun. 2015 Oct 1;6:8381. doi: 10.1038/ncomms9381. PMID:26423004[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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