1av6: Difference between revisions

Revision as of 10:43, 2 May 2008

VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE

OverviewOverview

Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nucleic acid backbone are three crucial and ubiquitous events in eukaryotic nucleic acid processing and function. These three events occur concurrently in the modification of vaccinia transcripts by the methyltransferase VP39. We report the crystal structure of a ternary complex comprising VP39, coenzyme product S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer. This structure reveals a novel and general mechanism for sequence-non-specific recognition of the mRNA transcript in which the protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and detailed view of a protein complexed with single-stranded RNA or 5'-capped mRNA.

About this StructureAbout this Structure

1AV6 is a Single protein structure of sequence from Vaccinia virus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme., Hodel AE, Gershon PD, Quiocho FA, Mol Cell. 1998 Feb;1(3):443-7. PMID:9660928 Page seeded by OCA on Fri May 2 10:43:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1av6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1av6 |SIZE=350|CAPTION= <scene name='initialview01'>1av6</scene>, resolution 2.800&Aring;
+The line below this paragraph, containing "STRUCTURE_1av6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5&#39;-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1av6|  PDB=1av6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1av6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1av6 OCA], [http://www.ebi.ac.uk/pdbsum/1av6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1av6 RCSB]</span>
-}}
 '''VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE'''
@@ Line 29: / Line 26: @@
 [[Category: Hodel, A E.]]
 [[Category: Quiocho, F A.]]
-[[Category: complex (transferase/rna)]]
+[[Category: Methyltransferase]]
-[[Category: methyltransferase]]
+[[Category: Mrna processing]]
-[[Category: mrna processing]]
+[[Category: Rna cap]]
-[[Category: poly(a) polymerase]]
+[[Category: Single-stranded rna]]
-[[Category: rna cap]]
+[[Category: Transcription]]
-[[Category: single-stranded rna]]
+[[Category: Vaccinia]]
-[[Category: transcription]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:43:56 2008''
-[[Category: vaccinia]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:48:15 2008''