1ark: Difference between revisions
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'''SH3 DOMAIN FROM HUMAN NEBULIN, NMR, 15 STRUCTURES''' | '''SH3 DOMAIN FROM HUMAN NEBULIN, NMR, 15 STRUCTURES''' | ||
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[[Category: Pastore, A.]] | [[Category: Pastore, A.]] | ||
[[Category: Politou, A S.]] | [[Category: Politou, A S.]] | ||
[[Category: | [[Category: Actin-binding]] | ||
[[Category: | [[Category: Nebulin]] | ||
[[Category: | [[Category: Sh3 domain]] | ||
[[Category: | [[Category: Transferase]] | ||
[[Category: | [[Category: Z-disk assembly]] | ||
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Revision as of 10:37, 2 May 2008
SH3 DOMAIN FROM HUMAN NEBULIN, NMR, 15 STRUCTURES
OverviewOverview
The huge modular protein nebulin is located in the thin filament of striated muscle in vertebrates and is thought to bind and stabilize F-actin. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain, the first of such motifs to be identified in a myofibrillar protein. We have determined the nebulin SH3 sequence from several species and found it strikingly conserved. We have also shown that the SH3 transcripts are constitutively expressed in skeletal muscle tissues. As the first step towards a molecular understanding of nebulin's cellular role we have determined the three-dimensional structure of the human nebulin SH3 domain in solution by nuclear magnetic resonance (NMR) spectroscopy and compared it with other known SH3 structures. The nebulin SH3 domain has a well-defined structure in solution with a typical SH3 topology, consisting of a beta-sandwich of two triple-stranded, antiparallel beta-sheets arranged at right angles to each other and of a single turn of a 310-helix. An additional double-stranded antiparallel beta-sheet in the RT loop bends over the beta-sandwich. The derived structure reveals a remarkable similarity with a distinct subset of SH3 domains, especially in the structural features of the exposed hydrophobic patch that is thought to be the site of interaction with polyproline ligands. On the basis of this similarity, we have modelled the interaction with an appropriate polyproline ligand and attempted to delineate the characteristics of the physiological SH3-binding partner in the Z-disk. Our results represent the first step in reconstructing the structure of nebulin and are expected to contribute to our understanding of nebulin's functional role in myofibrillar assembly.
About this StructureAbout this Structure
1ARK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
SH3 in muscles: solution structure of the SH3 domain from nebulin., Politou AS, Millevoi S, Gautel M, Kolmerer B, Pastore A, J Mol Biol. 1998 Feb 13;276(1):189-202. PMID:9514727 Page seeded by OCA on Fri May 2 10:37:19 2008