5b43: Difference between revisions
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==Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA== | ==Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA== | ||
<StructureSection load='5b43' size='340' side='right' caption='[[5b43]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='5b43' size='340' side='right'caption='[[5b43]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5b43]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B43 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5b43]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Acisb Acisb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B43 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b43 OCA], [http://pdbe.org/5b43 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b43 RCSB], [http://www.ebi.ac.uk/pdbsum/5b43 PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cpf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111120 ACISB])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b43 OCA], [http://pdbe.org/5b43 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b43 RCSB], [http://www.ebi.ac.uk/pdbsum/5b43 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b43 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5b43" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5b43" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[CRISPR type V|CRISPR type V]] | |||
*[[CRISPR-Cas|CRISPR-Cas]] | |||
*[[CRISPR-Cas Part II|CRISPR-Cas Part II]] | |||
*[[Endonuclease 3D structures|Endonuclease 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Acisb]] | |||
[[Category: Large Structures]] | |||
[[Category: Hirano, H]] | [[Category: Hirano, H]] | ||
[[Category: Ishitani, R]] | [[Category: Ishitani, R]] | ||
Revision as of 11:46, 11 March 2020
Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNACrystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA
Structural highlights
Function[CPF1_ACISB] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Has dsDNA endonuclease activity, results in a 4-base 5' overhang 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).[UniProtKB:A0Q7Q2][1] Publication Abstract from PubMedCpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) in complex with the guide RNA and its target DNA at 2.8 A resolution. AsCpf1 adopts a bilobed architecture, with the RNA-DNA heteroduplex bound inside the central channel. The structural comparison of AsCpf1 with Cas9, a type II CRISPR-Cas nuclease, reveals both striking similarity and major differences, thereby explaining their distinct functionalities. AsCpf1 contains the RuvC domain and a putative novel nuclease domain, which are responsible for cleaving the non-target and target strands, respectively, and for jointly generating staggered DNA double-strand breaks. AsCpf1 recognizes the 5'-TTTN-3' protospacer adjacent motif by base and shape readout mechanisms. Our findings provide mechanistic insights into RNA-guided DNA cleavage by Cpf1 and establish a framework for rational engineering of the CRISPR-Cpf1 toolbox. Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA.,Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O Cell. 2016 May 5;165(4):949-62. doi: 10.1016/j.cell.2016.04.003. Epub 2016 Apr, 21. PMID:27114038[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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