6k2h: Difference between revisions

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-'''Unreleased structure'''
-The entry 6k2h is ON HOLD  until Paper Publication
+==structural characterization of mutated NreA protein in nitrate binding site from staphylococcus aureus.==
+<StructureSection load='6k2h' size='340' side='right'caption='[[6k2h]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6k2h]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K2H FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2h OCA], [http://pdbe.org/6k2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k2h RCSB], [http://www.ebi.ac.uk/pdbsum/6k2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2h ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Staphylococcus aureus is an anaerobic facultative microorganism that features the NreABC system for nitrate respiration. NreB is the sensor histidine kinase that phosphorylates the response regulator NreC to stimulate the expression of target genes. NreA is a nitrate sensor which dissociates from NreB in the present of nitrate and relieves its inhibition on NreB. However, the molecular basis of how NreA regulate NreB remains unknown. In this study, we determined the crystal structures of nitrate-bound NreA from S. aureus (SaNreA/NO3(-)) and its apoNreA-like mutant SaNreAY(94A) in complex with ethanediol (SaNreA(Y94A)/EDO). Structural comparison reveals that the C-terminal loop in SaNreA/NO3(-) rearranges to an alpha-helix (alpha7) in SaNreA(Y94A)/EDO, which converts an acidic pocket on the surface to a positively charged region. This conformational change of SaNreA C-terminus might play a role in SaNreB binding.
-Authors: Sangare, L., Chen, W., Wang, C., Chen, X., Wu, M., Zhang, X., Zang, J.
+Structural insights into the conformational change of Staphylococcus aureus NreA at C-terminus.,Sangare L, Chen W, Wang C, Chen X, Wu M, Zhang X, Zang J Biotechnol Lett. 2020 Jan 22. pii: 10.1007/s10529-020-02807-2. doi:, 10.1007/s10529-020-02807-2. PMID:31970556<ref>PMID:31970556</ref>
-Description: structural characterization of mutated NreA protein in nitrate binding site from staphylococcus aureus.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6k2h" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Chen, W]]
+[[Category: Chen, X]]
+[[Category: Sangare, L]]
+[[Category: Wang, C]]
+[[Category: Wu, M]]
 [[Category: Zang, J]]
-[[Category: Chen, X]]
 [[Category: Zhang, X]]
-[[Category: Wu, M]]
+[[Category: Complex]]
-[[Category: Wang, C]]
+[[Category: Signaling protein]]
-[[Category: Chen, W]]
-[[Category: Sangare, L]]