6c66

CRISPR RNA-guided surveillance complex, pre-nickingCRISPR RNA-guided surveillance complex, pre-nicking

6c66, resolution 3.66Å Show:Asymmetric Unit Biological Assembly Structural highlights 6c66 is a 15 chain structure with sequence from [1] and Thefy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Gene: Tfu_1593 (THEFY), Tfu_1592 (THEFY), Tfu_1590 (THEFY), Tfu_1591 (THEFY), Tfu_1589 (THEFY), Tfu_1588 (THEFY) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA, by the RNA-guided Cascade complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here we present a 3.7 A resolution cryo-EM structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the non-target strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7 A resolution cryo-EM structure captures the post-nicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for ATP-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems. Structure basis for RNA-guided DNA degradation by Cascade and Cas3.,Xiao Y, Luo M, Dolan AE, Liao M, Ke A Science. 2018 Jun 7. pii: science.aat0839. doi: 10.1126/science.aat0839. PMID:29880725 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRISPR type I-E (Cascade) Helicase 3D structures