6l7h: Difference between revisions
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<StructureSection load='6l7h' size='340' side='right'caption='[[6l7h]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='6l7h' size='340' side='right'caption='[[6l7h]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6l7h]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L7H FirstGlance]. <br> | <table><tr><td colspan='2'>[[6l7h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glygl Glygl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L7H FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E7F:1-[3-[(2S,3R,4R,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]-2,4,6-tris(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one'>E7F</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E7F:1-[3-[(2S,3R,4R,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]-2,4,6-tris(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one'>E7F</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l7h OCA], [http://pdbe.org/6l7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l7h RCSB], [http://www.ebi.ac.uk/pdbsum/6l7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l7h ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l7h OCA], [http://pdbe.org/6l7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l7h RCSB], [http://www.ebi.ac.uk/pdbsum/6l7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l7h ProSAT]</span></td></tr> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Glygl]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Li, F D]] | [[Category: Li, F D]] | ||
Revision as of 10:11, 4 March 2020
crystal structure of GgCGT in complex with UDP and Nothofagincrystal structure of GgCGT in complex with UDP and Nothofagin
Structural highlights
Publication Abstract from PubMedA highly efficient di-C-glycosyltransferase GgCGT was discovered from the medicinal plant Glycyrrhiza glabra. GgCGT catalyzes a two-step di-C-glycosylation of flopropione-containing substrates with conversion rates of >98%. To elucidate the catalytic mechanisms of GgCGT, we solved its crystal structures in complex with UDP-Glc, UDP-Gal, UDP/phloretin, and UDP/nothofagin, respectively. Structural analysis revealed that the sugar donor selectivity was controlled by the hydrogen-bond interactions of sugar hydroxyl groups with D390 and other key residues. The di-C-glycosylation capability of GgCGT was attributed to a spacious substrate-binding tunnel, and the G389K mutation could switch di- to mono-C-glycosylation. GgCGT is the first di-C-glycosyltransferase with a crystal structure, and the first C-glycosyltransferase with a complex structure containing a sugar acceptor. This work could benefit the development of efficient biocatalysts to synthesize C-glycosides with medicinal potential. Functional Characterization and Structural Basis of an Efficient Di-C-glycosyltransferase from Glycyrrhiza glabra.,Zhang M, Li FD, Li K, Wang ZL, Wang YX, He JB, Su HF, Zhang ZY, Chi CB, Shi XM, Yun CH, Zhang ZY, Liu ZM, Zhang LR, Yang DH, Ma M, Qiao X, Ye M J Am Chem Soc. 2020 Feb 11. doi: 10.1021/jacs.9b12211. PMID:31986016[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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