2bn5: Difference between revisions
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==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide== | ==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide== | ||
<StructureSection load='2bn5' size='340' side='right' caption='[[2bn5]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''> | <StructureSection load='2bn5' size='340' side='right'caption='[[2bn5]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BN5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BN5 FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Nucleoprotein|Nucleoprotein]] | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Drome]] | [[Category: Drome]] | ||
[[Category: Large Structures]] | |||
[[Category: Butler, P J.G]] | [[Category: Butler, P J.G]] | ||
[[Category: Ignjatovic, T]] | [[Category: Ignjatovic, T]] | ||
Revision as of 11:06, 19 February 2020
P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptideP-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide
Structural highlights
Function[RU17_DROME] Mediates the splicing of pre-mRNA by binding to the stem loop I region of U1-snRNA.[1] Publication Abstract from PubMedP-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein. Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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