6sy9: Difference between revisions
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<StructureSection load='6sy9' size='340' side='right'caption='[[6sy9]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='6sy9' size='340' side='right'caption='[[6sy9]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6sy9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SY9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SY9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6sy9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SY9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SY9 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sy9 OCA], [http://pdbe.org/6sy9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sy9 RCSB], [http://www.ebi.ac.uk/pdbsum/6sy9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sy9 ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C3926_14995, C3927_13800, D7214_05345, ERS240541_01816, ERS253249_00465, NCTC12000_02932 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sy9 OCA], [http://pdbe.org/6sy9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sy9 RCSB], [http://www.ebi.ac.uk/pdbsum/6sy9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sy9 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The water-borne bacterium Legionella pneumophila replicates in environmental protozoa and upon inhalation destroys alveolar macrophages, thus causing a potentially fatal pneumonia termed 'Legionnaires' disease'. L. pneumophila employs the Legionella quorum sensing (Lqs) system to control its life cycle, pathogen-host cell interactions, motility and natural competence. Signaling through the Lqs system occurs through the alpha-hydroxyketone compound Legionella autoinducer-1 (LAI-1) and converges on the prototypic response regulator LqsR, which dimerizes upon phosphorylation of the conserved aspartate, D108 . In this study, we determine the high-resolution structure of monomeric LqsR. The structure reveals a receiver domain adopting a canonical (betaalpha)5 fold, which is connected through an additional sixth helix and an extended alpha5-helix to a novel output domain. The two domains delineate a mainly positively charged groove, and the output domain adopts a five-stranded antiparallel beta-sheet fold similar to nucleotide-binding proteins. Structure-based mutagenesis identified amino acids critical for LqsR phosphorylation and dimerization. Upon phosphorylation, the LqsRD172A and LqsRD302N/E303Q mutant proteins dimerized even more readily than wild-type LqsR, and no evidence for semi-phosphorylated heterodimers was obtained. Taken together, the high-resolution structure of LqsR reveals functionally relevant amino acid residues implicated in signal transduction of the prototypic response regulator. | |||
The structure of the Legionella response regulator LqsR reveals amino acids critical for phosphorylation and dimerization.,Hochstrasser R, Hutter CAJ, Arnold FM, Barlocher K, Seeger MA, Hilbi H Mol Microbiol. 2020 Jan 29. doi: 10.1111/mmi.14477. PMID:31997467<ref>PMID:31997467</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6sy9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 33152]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Arnold, F M]] | [[Category: Arnold, F M]] | ||