User:Nikhil Malvankar/Cytochrome nanowires: Difference between revisions

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Seamless micrometer-long polymerization of hundreds of cytochromes is without precedent, to the knowledge of the authors. The filaments whose structure was determined here were obtained from electrode-grown cells. However, fumarate-grown cells produced filaments with similar sinusoidal morphology. The purified OmcS filaments have morphology and power spectra similar to cell-attached filaments previously thought to be type IV pili. Direct current electrical conductivity of the wild type ~4 nm OmcS filaments was confirmed, and was comparable to previously reported filament conductivity values.

Cells with the ''omcS'' gene deleted (''&~~delta~~;omcS'') produced thinner (~1.7 nm) filaments which were smooth (not sinusoidal) and had electrical conductivity >100-fold lower than the OmcS filaments.

Cells with the ''omcS'' gene deleted (''ΔomcS'') produced thinner (~1.7 nm) filaments which were smooth (not sinusoidal) and had electrical conductivity >100-fold lower than the OmcS filaments.

Previous studies showed that PilA is required for export of OmcS. However, PilA was not found in the filaments studied here. Thus, PilA may be required for production of OmcS filaments, but not be a structural component of those filaments.

@@ Line 89: / Line 89: @@
 Seamless micrometer-long polymerization of hundreds of cytochromes is without precedent, to the knowledge of the authors. The filaments whose structure was determined here were obtained from electrode-grown cells. However, fumarate-grown cells produced filaments with similar sinusoidal morphology. The purified OmcS filaments have morphology and power spectra similar to cell-attached filaments previously thought to be type IV pili. Direct current electrical conductivity of the wild type ~4 nm OmcS filaments was confirmed, and was comparable to previously reported filament conductivity values.
-Cells with the ''omcS'' gene deleted (''&delta;omcS'') produced thinner (~1.7 nm) filaments which were smooth (not sinusoidal) and had electrical conductivity >100-fold lower than the OmcS filaments.
+Cells with the ''omcS'' gene deleted (''&Delta;omcS'') produced thinner (~1.7 nm) filaments which were smooth (not sinusoidal) and had electrical conductivity >100-fold lower than the OmcS filaments.
 Previous studies showed that PilA is required for export of OmcS. However, PilA was not found in the filaments studied here. Thus, PilA may be required for production of OmcS filaments, but not be a structural component of those filaments.