2avf: Difference between revisions
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==Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes== | ==Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes== | ||
<StructureSection load='2avf' size='340' side='right' caption='[[2avf]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='2avf' size='340' side='right'caption='[[2avf]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2avf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_cycloclastes"_(gray_and_thornton)_bergey_et_al. "achromobacter cycloclastes" (gray and thornton) bergey et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AVF FirstGlance]. <br> | <table><tr><td colspan='2'>[[2avf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_cycloclastes"_(gray_and_thornton)_bergey_et_al. "achromobacter cycloclastes" (gray and thornton) bergey et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AVF FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Nitrite reductase|Nitrite reductase]] | *[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: An, X M]] | [[Category: An, X M]] | ||
[[Category: Chang, T]] | [[Category: Chang, T]] | ||
Revision as of 08:21, 13 February 2020
Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastesCrystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMonoclinic crystal structure of C-terminal desundecapeptide nitrite reductase (NiRc-11) from Achromobacter cycloclastes was determined at 2.6A. NiRc-11 exists as a loose trimer in the crystal. Deletion of 11 residues eliminates all intersubunit hydrogen bonds mediated by the C-terminal tail. The rigid irregular coil 105-112, which constitutes part of the sidewall of the active site pocket, undergoes conformational changes and becomes highly flexible in NiRc-11. Correspondingly, the linker segments between the two copper sites 95-100 and 135-136 are partly relaxed in conformation, which leads to disrupted active site microenvironments responsible for the activity loss and spectral change of NiRc-11. Comparison with the native structure revealed a bulky residue Met331 fastened by hydrogen bonding, which may play a direct role in keeping the right copper site geometry by protruding its side chain against the irregular coil 105-112. Sequence alignment showed that the bulky residue is conserved at position 331, indicating an equal importance of C-terminal segment in other copper-containing nitrite reductases. Crystal structure of C-terminal desundecapeptide nitrite reductase from Achromobacter cycloclastes.,Li HT, Chang T, Chang WC, Chen CJ, Liu MY, Gui LL, Zhang JP, An XM, Chang WR Biochem Biophys Res Commun. 2005 Dec 30;338(4):1935-42. Epub 2005 Nov 15. PMID:16293231[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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